| pcr amplification and sequence of the p33 piroplasm surface antigen gene of a theileria species isolated from cattle in west java, indonesia. | oriental theileriosis, a parasitic disease of cattle caused by protozoa of the theileria orientalis/sergenti/buffeli group, has been reported in indonesia but its causal agent had not yet been characterized. this study was carried out to isolate and characterize the parasite through comparison of its p33 piroplasm surface antigen gene sequence, with known p32 sequences of t. sergenti and t. buffeli isolates. a theileria spp. isolate was collected from an ongole cow in jonggol, west-java, and tra ... | 1998 | 9668457 |
| characterisation of the 33kda piroplasm surface antigen of theileria orientalis/sergenti/buffeli isolates from west java, indonesia. | the immunodominant 33/35kda antigen of a theileria isolate from west java, indonesia, was characterised and immuno-affinity purified by use of a monoclonal antibody, kul-a4, and was shown to be representative of the t. orientalis/sergenti/buffeli group. the aminoterminal sequence of the purified 35kda peptide (20 residues) was determined by automated edman degradation and found to correspond to the predicted amino acid sequence of a prospective p33 gene previously sequenced from the same isolate ... | 2002 | 11809330 |