| prevalence of equine piroplasmosis and its association with tick infestation in the state of são paulo, brazil. | serum samples were collected from 582 horses from 40 stud farms in the state of são paulo and tick (acari: ixodidae) infestations were evaluated on them. serum samples were subjected to the complement fixation test (cft) and a competitive inhibition elisa (celisa) for babesia caballi and theileria equi. logistic regression analyses were performed to construct multivariate models that could explain the dependent variable (horses positive for b. caballi or t. equi) as a function of the independent ... | 2009 | 20040201 |
| occurrence of theileria equi in horses raised in the jaboticabal microregion, são paulo state, brazil. | blood and serum samples from 170 horses raised in the jaboticabal microregion, são paulo state, brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (ifat) and nested polymerase chain reaction (npcr) for theileria equi infections. the association among the test results was verified by the mcnemar test. during the examination of thin blood smears, parasites were detected in six (3.52%) horses. anti-t. equi antibodies were detected in 100 ... | 2010 | 21184699 |
| production of recombinant ema-1 protein and its application for the diagnosis of theileria equi using an enzyme immunoassay in horses from s+úo paulo state, brazil. | the erythrocytic-stage surface protein, equi merozoite antigen 1 (ema-1), is a major candidate for the development of a diagnostic antigen for equine piroplasmosis. in order to establish an effective diagnostic method for practical use, the gene encoding the entire ema-1 of theileria equi jaboticabal strain was cloned and expressed in escherichia coli as a histidine-tagged protein (his6-ema1). the expressed ema-1 reacted with specific antibodies in western blot and had an apparent molecular mass ... | 2011 | 21439233 |
| molecular and serological detection of theileria equi and babesia caballi in donkeys (equus asinus) in brazil. | piroplasmosis in donkeys has been recognized as a serious problem of major economic importance, since the affected animals manifest loss of appetite and decreased working capacity. the present work is aimed at detecting infection or exposure of donkeys in são paulo, brazil to theileria (t.) equi and babesia (b.) caballi using molecular and serological approaches. edta-blood and serum samples were collected from 88 donkeys (equus asinus). from 88 sampled donkeys, 65 (73.86%; 95% confidence interv ... | 2011 | 22186194 |