| lyme disease spirochetes in japan: enzootic transmission cycles in birds, rodents, and ixodes persulcatus ticks. | the ixodid tick, ixodes persulcatus, serves as a vector of borrelia species associated with lyme disease in hokkaido, japan. the migratory birds of genera emberiza and turdus and the woodland rodents of genera apodemus and clethrionomys are the wildlife reservoirs. to systemize the enzootic transmission cycles, borreliae isolates were classified by ribosomal rna gene restriction fragment length polymorphism (rflp) analysis. most (> 60%) isolates from the bird-feeding i. persulcatus larvae belong ... | 1994 | 7930730 |
| borrelia burgdorferi sensu lato in an endemic environment: wild sika deer (cervus nippon yesoensis) with infected ticks and antibodies. | ticks and blood samples were collected from wild sika deer (cervus nippon yesoensis) during a hunting season (august to october) of 1991 at a selected location in hokkaido, japan. ixodes persulcatus (adult and nymph) and i. ovatus (adult) were the common ticks on sika deer. spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. by the reactive pattern of monoclonal antibodies, isolates we ... | 1996 | 8871523 |
| a phylogenetic analysis of borrelia burgdorferi sensu lato isolates associated with lyme disease in japan by flagellin gene sequence determination. | we determined nearly complete flagellin gene sequences for borrelia burdgdorferi sensu lato isolates (11 isolates obtained from ixodes persulcatus ticks and patients in hokkaido, japan, and 1 european isolate) representing six different restriction fragment length polymorphism (rflp) ribotype groups following cloning of the pcr-amplified genes. these sequences were aligned with those of representatives of the three borrelia species associated with lyme disease, and a phylogenetic tree was constr ... | 1996 | 8934900 |
| genetic diversity and the absence of regional differences of borrelia garinii as demonstrated by ospa and ospb gene sequence analysis. | unfed adult ixodes persulcatus ticks were collected from four locations of nagano and hokkaido in japan. infected borrelia garinii were investigated by pcr-rflp of the ospa and ospb gene sequences. the primer set amplified an approximately 1.6-kb dna fragment (0.7-kb in some strains), and bsri, bstyi, or nlaiii digestion of the product resulted in six distinctively different pcr-rflp groups and two independent borrelial strains. the representatives in each pcr-rflp group and individuals from the ... | 1999 | 10656177 |
| western blot analysis for diagnosis of lyme disease in acute facial palsy. | lyme borreliosis has been implicated in the pathogenesis of acute peripheral facial palsy (apfp). few studies, however, have used western blot analyses to confirm the serological diagnosis. | 2001 | 11359146 |
| detection of borrelia garinii, borrelia tanukii and borrelia sp. closely related to borrelia valaisiana in ixodes ticks removed from dogs and cats in japan. | ticks removed from 1136 dogs and 134 cats all over japan were examined for borrelia infection by pcr and sequencing. the 5s-23s rdna intergenic spacer of borrelia was detected from two ixodes persulcatus ticks from two dogs and two unidentified ixodes spp. from another two dogs in hokkaido, and two ixodes granulatus ticks from two cats in okinawa. additional 2 i. granulatus from the same cats also showed positive. sequence analysis of the pcr products revealed that the one from hokkaido was simi ... | 2007 | 17049744 |
| serological survey of five zoonoses, scrub typhus, japanese spotted fever, tularemia, lyme disease, and q fever, in feral raccoons (procyon lotor) in japan. | we investigated the seroprevalence of five tick- or mite-borne zoonoses, scrub typhus (orientia tsutsugamushi), japanese spotted fever (rickettsia japonica), tularemia (francisella tularensis), lyme disease (borrelia afzelii and borrelia garinii), and q fever (coxiella burnetii), in feral raccoons (procyon lotor) captured in hokkaido and kanagawa prefectures in japan. of the 559 raccoons captured in hokkaido, 8 (1.4%), 3 (0.5%), 1 (0.2%), and 1 (0.2%) carried antibodies against o. tsutsugamushi ... | 2011 | 20553108 |
| prevalence of lyme borrelia in ixodes persulcatus ticks from an area with a confirmed case of lyme disease. | in this study, the prevalence of borrelia infections in ixodes ticks from a site in hokkaido, japan, with confirmed cases of lyme disease was determined by a pcr method capable of detecting and differentiating between strains of pathogenic borrelia, with particular emphasis on borrelia garinii (b. garinii) and borrelia afzelli (b. afzelli), using tick-derived dna extracts as template. a total of 338 ticks, inclusive of 284 ixodes persulcatus (i. persulcatus), were collected by flagging vegetatio ... | 2013 | 23047332 |
| survey on tick-borne pathogens in thoroughbred horses in the hidaka district, hokkaido, japan. | a total of 87 thoroughbred horses and 10 ixodid ticks from a ranch in hidaka district, hokkaido were tested for tick-borne diseases. using the indirect fluorescent antibody (ifa) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥ 80 against anaplasma phagocytophilum, rickettsia helvetica, and borrelia garinii, respectively. this is the first report of infection with the 3 pathogens in horses in japan. using pcr, dnas from the peripheral blood of all horses were found negative ... | 2013 | 22878540 |
| borrelia miyamotoi infections among wild rodents show age and month independence and correlation with ixodes persulcatus larval attachment in hokkaido, japan. | to clarify how borrelia miyamotoi is maintained in the environment in hokkaido, we examined ixodes persulcatus for its prevalence among wild rodents and its tick vector by detecting a portion of the borrelial flab gene in rodent urinary bladder and blood samples, and from whole ticks. we compared b. miyamotoi infection rates to borrelia garinii and borrelia afzelii, which are human lyme disease pathogens also carried by wild rodents, and which are transmitted by the same vector tick. whereas b. ... | 2013 | 23210636 |
| differential tick burdens may explain differential borrelia afzelii and borrelia garinii infection rates among four, wild, rodent species in hokkaido, japan. | the ecologies of lyme disease borrelia spp. are very specific to location, as they are dependent upon the spirochete species and genotypes, the vectors and the host vertebrates present. in hokkaido, japan, where two human pathogenic, lyme disease borrelia spp. are present, and human cases are reported annually, the ecologies have been poorly studied. our goal was to determine whether variation in borrelial infection rates among rodent species sharing an environment, is due to immunological or ec ... | 2013 | 23391510 |
| astasia and pyrexia related to borrelia garinii infection in two dogs in hokkaido, japan. | two dogs that exhibited sudden astasia, anorexia and fever higher than 40°c were suspected of having lyme disease in july 2011. clinical symptoms gradually improved with antibiotic treatment in both cases. polymerase chain reaction and sequence analysis revealed borrelia garinii dna fragments in the peripheral blood in the acute disease phase. serological tests, including enzyme linked immunosorbent assay and western blot analysis, showed an increased igg antibody titer against borrelia pathogen ... | 2013 | 23428777 |