isolation, identification, and pathogenicity of two field strains of infectious bursal disease virus.using a sentinel bird approach, two field isolates of infectious bursal disease virus (ibdv) were isolated from broiler farms in two major broiler-producing areas of the state of georgia. these farms had a history of subclinical ibd associated with respiratory problems and poor performance. isolates designated as u-28 and 3212 were isolated using specific-pathogen-free chicken embryos and chicken embryo bursal cells. these isolates were identified by means of agar gel precipitation and virus-neu ...19892539074
sequence comparisons of the variable vp2 region of eight infectious bursal disease virus isolates.the vp2 gene is part of the genomic segment a of infectious bursal disease virus (ibdv). it has been identified as the major host-protective antigen of ibdv and is known to contain conformationally dependent protective epitopes. a 643-base pair segment covering the hypervariable region of this gene from three recent serologic variant ibdv isolates from the southeastern united states, two variants from the delmarva peninsula, and three serologic standard viruses were amplified and sequenced using ...19979087318
survey for antibodies to infectious bursal disease virus serotype 2 in wild turkeys and sandhill cranes of florida, usa.captive-reared whooping cranes (grus americana) released into florida for the resident reintroduction project experienced unusually high mortality and morbidity during the 1997-98 and 2001-02 release seasons. exposure to infectious bursal disease virus (ibdv) serotype 2 as evidenced by seroconversion was suspected to be the factor that precipitated these mortality events. very little is known about the incidence of ibd in wild bird populations. before this study, natural exposure had not been do ...201020688680
viral competition and maternal immunity influence the clinical disease caused by very virulent infectious bursal disease virus.the very virulent form of infectious bursal disease virus (vvibdv) causes an immunosuppressive disease that is further characterized by the rapid onset of morbidity and high mortality in susceptible chickens. in 2009, vvibdv was first reported in california, u. s. a., and since that time only a few cases of acute infectious bursal disease attributed to vvibdv have been recognized in california. in other countries where vvibdv has become established, it rapidly spreads to most poultry-producing r ...201122017037
molecular characterization of infectious bursal disease virus from commercial poultry in the united states and latin america.from june 1999 to september 2001, 216 bursal samples from broiler farms in the united states and from countries of latin america were submitted to the poultry diagnostic and research center at the university of georgia for the purpose of genotyping field infectious bursal disease viruses (ibdvs). the reverse transcriptase-polymerase chain reaction (rt-pcr) was used to amplify a 248-bp product, encompassing the hypervariable region of vp2 gene. the genotyping was conducted by restriction fragment ...200312713162
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