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sequence and phylogenetic analysis of a chinese very virulent infectious bursal disease virus.the complete genome sequence of a chinese very virulent infectious bursal disease virus (vvibdv) strain, harbin-1, was determined. based on the sequence analysis, the molecular characteristics and potential virulence determinants and origin of vvibdv strains were identified. phylogenetic analysis indicated that a reassortment and/or recombination event may have occurred in the emergence of chinese vvibdv strains.200818622570
molecular characteristics of full-length genomic segment a of three infectious bursal disease viruses in china: two attenuated strains and one virulent field strain.the full-length cdna of genomic segment a of three infectious bursal disease viruses, two attenuated strains (hz2 and jd1) and one virulent field strain (zj2000), was amplified in a single step by reverse transcription-polymerase chain reaction, cloned into pgem-t easy vector, and sequenced. the full length of cloned segment a contains 3259 nucleotides, which includes two partially overlapping open reading fragments (orfs) orf1 and orf2, flanked by 5' and 3' noncoding regions. these strains shar ...200111785891
assessment of genetic, antigenic and pathotypic criteria for the characterization of ibdv strains.the aim of this work was the selection and comparison of representative infectious bursal disease virus (ibdv) strains. nine strains of ibdv, isolated at different times and from different geographic regions of europe and china, were characterized. batches of all strains were prepared following standardized protocols and checked for the absence of contaminating viruses. criteria used for their characterization were: (i) the nucleotide sequence of the vp2 variable region, (ii) binding to a panel ...200415545026
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