sequence and analysis of genomic segment a and b of very virulent infectious bursal disease virus isolated from china.a very virulent infectious bursal disease virus (vvibdv) field strain, named sh95, was identified and characterized from flocks with vaccination failure in shanghai. the use of random primer and a reverse transcriptase lacking rnase-h activity produced full-length cdna copies of the viral genomic a and b segments of sh95. the 3259 base pairs (bp) of segment a and 2827 bp of segment b were amplified by long and accurate pcr in a single step, then successfully cloned and sequenced. there were five ...200312667193
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