| sequence and analysis of genomic segment a and b of very virulent infectious bursal disease virus isolated from china. | a very virulent infectious bursal disease virus (vvibdv) field strain, named sh95, was identified and characterized from flocks with vaccination failure in shanghai. the use of random primer and a reverse transcriptase lacking rnase-h activity produced full-length cdna copies of the viral genomic a and b segments of sh95. the 3259 base pairs (bp) of segment a and 2827 bp of segment b were amplified by long and accurate pcr in a single step, then successfully cloned and sequenced. there were five ... | 2003 | 12667193 |