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molecular characterisation of very virulent infectious bursal disease viruses in taiwan.the very virulent infectious bursal disease virus (vv ibdv) rna in the bursa of fabricius and spleen from experimentally infected chickens or field samples was detected by in situ hybridisation (ish) with subsequent reverse transcription (rt)-polymerase chain reaction (pcr) and sequence analysis. the vp 2 gene of vv ibdv was detected by ish in infected chicken tissues with a cloned digoxigenin (dig)-labelled cdna probe. to verify ish, rt - pcr was used to amplify two 643- and 500-base pair fragm ...200111356093
immunogenicity and antigenicity of very virulent strains of infectious bursal disease viruses.the immunogenicity and antigenicity of three very virulent (vv) strains of infectious bursal disease viruses (ibdvs) from turkey (oa), holland (hol), and taiwan (pt) were investigated, and their antigenic relationships with the american classic sal, variant in serotype 1, and serotype 2 oh ibdvs were studied. in the present study, the vvibdvs were passaged eight times in chicken embryos and were characterized by reverse transcription/polymerase chain reaction-restriction fragment length polymorp ...200111332505
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