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genotyping of trichophyton rubrum by analysis of ribosomal-dna intergenic spacer regions.an attempt was made to explore the genotyping of trichophyton rubrum (t. rubrum) and the relationship between genotype and geographical origin using ribosomal restriction endonuclease polymorphic analysis. the total dna was extracted by cetyltrimethyl ammonium bromide (ctab). the probe was amplified from part of the 18s, itsi, 5.8s, and itsii region of t. rubrum standard strain with the universal fungal primers ns5 [5'-aactt aaagg aattg acgga ag-3'] and its4 [5'-tcctc cgctt attga tatgc-3']. the ...200717554639
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