cutaneous leishmaniasis caused by members of leishmania braziliensis complex in nayarit, state of epidemiological study was carried out in the northern mexican state, nayarit. fourteen patients with possible cutaneous leishmaniasis skin lesions gave positive montenegro skin tests. biopsies were taken from the skin ulcer and analyzed by polymerase chain reaction (pcr) with specific primers for the leishmania mexicana complex; however all biopsies were not amplified. pcr carried out with specific primers for the l. braziliensis complex resulted in the amplification of all patient dna. dna f ...200111285471
molecular probes and the polymerase chain reaction for detection and typing of leishmania species in mexico.leishmaniasis in mexico is a public health problem because all the clinical forms have been recorded in most mexican states. we studied patients showing clinical symptoms of any form of leishmaniasis, from several endemic areas. bone marrow samples, aspirates or skin biopsies were taken and deoxyribonucleic acid (dna) was extracted and amplified by the polymerase chain reaction (pcr) with universal primers ajs1 and deb8, specific for the leishmania subgenus leishmania. the pcr products were then ...200212055821
molecular diagnosis of leishmania mexicana in a cutaneous leishmaniasis case in sinaloa, mexico.abstract leishmaniasis has been considered endemic in sinaloa, mexico, since 1994. despite that leishmania mexicana is the main etiological agent of cutaneous leishmaniasis (cl) in other regions of mexico, the species causing cl in patients from sinaloa state has not been previously established, although leishmania braziliensis has been found in the neighboring southern state, nayarit. l. braziliensis is also associated with mucocutaneous leishmaniasis, which is a more complicated clinical var ...201121923263
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