| putative leishmania hybrids in the eastern andean valley of huanuco, peru. | during an outbreak of tegumentary leishmaniasis that developed in the 1990s in the eastern andean valley of huanuco, peru, the coexistence of andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. seven human isolates of leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic dna and molecular karyotyping. the three methods identified 3 isolates as l. braziliensis, and ... | 1995 | 8533665 |
| the transmission dynamics of canine american cutaneous leishmaniasis in huánuco, peru. | the epidemiology of canine american cutaneous leishmaniasis (acl) due to leishmania (viannia) spp. was investigated in huánuco, peru to 1) describe the natural course of canine l. (viannia) infections and 2) assess the role of domestic dogs as acl reservoir hosts. over a three-year period 1,022 dogs were surveyed, with cumulative village l. (viannia) prevalence being 26% (range = 0-100%). the incidence of l. (viannia) was estimated to be 0.285 dogs/year (95% confidence interval [ci] = 0.160-0.41 ... | 2003 | 14695083 |
| identification of causative leishmania species in giemsa-stained smears prepared from patients with cutaneous leishmaniasis in peru using pcr-rflp. | a pcr-restriction fragment length polymorphism (rflp) targeting the mannose phosphate isomerase gene was established to differentiate leishmania species distributed near the department of huanuco, peru. the technique was applied to 267 dna samples extracted from giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative leishmania species was identified. of 114 pcr-amplified samples, 22, 19, 24 and 49 samp ... | 2016 | 26943992 |
| first evidence of a hybrid of leishmania (viannia) braziliensis/l. (v.) peruviana dna detected from the phlebotomine sand fly lutzomyia tejadai in peru. | the natural infection of sand flies by leishmania was examined in the department of huanuco of peru, where cutaneous leishmaniasis caused by a hybrid of leishmania (viannia) braziliensis/l. (v.) peruviana is endemic. a total of 2,997 female sand flies were captured by cdc light traps and shannon traps, of which 2,931 and 66 flies were identified as lutzomyia tejadai and lu fischeri, respectively. using crude dna extracted from individual sand flies as a template, leishmania dna was detected from ... | 2016 | 26735142 |
| domestic dog ownership: a risk factor for human infection with leishmania (viannia) species. | an epidemiological study has shown that cumulative, village prevalence of leishmania (viannia) infection in dogs ranges from 8% to 45% in huánuco, peru. using data from a prospective survey of human american cutaneous leishmaniasis (acl) collected during 1994-98, it was shown that the village-level risk of human acl did not significantly increase with dog abundance, neither in absolute terms (p = 0.659) nor in relation to dog:human ratios (p = 0.213). a significant positive association was obser ... | 2003 | 14584365 |
| in vitro promastigote fitness of putative leishmania (viannia) braziliensis/leishmania (viannia) peruviana hybrids. | in order to initiate studies on the phenotypic properties of hybrids vs. their putative parents, the in vitro growth behaviour of promastigotes was compared for 15 stocks characterised as leishmania (viannia) braziliensis, leishmania (viannia) peruviana and putative hybrids (isolated from the eastern andean valley of huanuco, peru). five sets of three stocks, each set including a l.(v.)braziliensis, a l.(v.)peruviana and a putative hybrid, were constituted randomly and counted daily close to iso ... | 1999 | 9924965 |