detection of leishmania braziliensis in human paraffin-embedded tissues from tucumán, argentina by polymerase chain reaction. | american cutaneous leishmaniasis (acl) is an endemic disease in northern argentina. we applied the polymerase chain reaction (pcr) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of tucumán, argentina. we used primers previously designed to detect a leishmania-specific 120-base-pair fragment of kinetoplast dna minicircle, other two primer pairs that amplify kdna minicircles b ... | 2005 | 16021307 |