detection of leishmania braziliensis in human paraffin-embedded tissues from tucumán, argentina by polymerase chain reaction.american cutaneous leishmaniasis (acl) is an endemic disease in northern argentina. we applied the polymerase chain reaction (pcr) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of tucumán, argentina. we used primers previously designed to detect a leishmania-specific 120-base-pair fragment of kinetoplast dna minicircle, other two primer pairs that amplify kdna minicircles b ...200516021307
natural infection of lutzomyia neivai with leishmania spp. in northwestern argentina.the natural infection of lutzomyia neivai with leishmania in the endemic area of american cutaneous leishmaniasis (acl) in northwestern argentina was analyzed by the polymerase chain reaction (pcr)-hybridization technique. phlebotominae sand flies were captured in the provinces of tucumán and salta between 1999 and 2003. from a sample of 440 lu. neivai females analysed for the detection of the leishmania (viannia) and leishmania (leishmania) subgenera, 9.1% of the samples resulted infected with ...200616529708
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