identification and distribution of new world leishmania species characterized by serodeme analysis using monoclonal antibodies.five hundred thirty stocks of leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of american cutaneous (acl) and visceral leishmaniases (avl) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. this study confirms and extends our preliminary results on the high specificity of some of these ...19873826486
a survey for american cutaneous and visceral leishmaniasis among 1,342 dogs from areas in rio de janeiro (brazil) where the human diseases occur.there are areas in the periphery of rio de janeiro city where human cases of visceral and/or cutaneous leishmaniasis occur. the parasites have been identified as leishmania donovani and leishmania braziliensis braziliensis respectively. a survey for leishmaniasis was done among 1,342 dogs from those areas using an indirect immunofluorescent test. from the dogs, 616 came from areas where only human cases of visceral leishmaniasis occurred, 373 from an area where all human cases were of cutaneous ...19853910994
[epidemiologic study on tegumentary leishmaniasis in the municipality of corguinho, mato grosso do sul -- studies in the human population].a study on the resident population of 150 inhabitants of boa sorte in the municipality of corguinho, mato grosso do sul state, brazil was made, from march 1991 to march 1994, to establish the prevalence of south american cutaneous leishmaniasis (sacl), and to characterize the affected population, in an area of recent transmission. twelve of the inhabitants showed lesions suspected to be sacl, and in 8 cases it was possible to confirm this by biopsy and parasitology. the mucosal form was found in ...19957480911
leishmania (viannia) braziliensis is the prevalent species infecting patients with tegumentary leishmaniasis from mato grosso state, brazil.the frequency of leishmania (viannia) braziliensis infection among patients of mato grosso, brazil was estimated by polymerase chain reaction-pcr, dna hybridization and by isoenzyme electrophoresis. analysis of dna polymorphism was carried out using random amplified polymorphic dna-pcr (rapdpcr) with five different primers. the patients were attended from may 1997 to february 1998 at the reference ambulatory for american tegumentary leishmaniasis at júlio müller university hospital of the federa ...200616846569
lutzomyia (nyssomyia) whitmani s.l . (antunes & coutinho, 1939)(diptera: psychodidae: phlebotominae): geographical distribution and the epidemiology of american cutaneous leishmaniasis in brazil--mini-review.the aim of the present study, in view of the widespread geographical distribution of lutzomyia (nyssomyia) whitmani s.l. in brazil, in close association with the regions of transmission of leishmania (viannia) braziliensis and l. (v.) shawi, is to investigate the distribution of this sand fly species and american cutaneous leishmaniasis (acl) in relationship to vegetation and landscape ecology throughout brazil. thematic maps were elaborated by the mapinfo programme, giving information on the sp ...200717426877
the finding of lutzomyia almerioi and lutzomyia longipalpis naturally infected by leishmania spp. in a cutaneous and canine visceral leishmaniases focus in serra da bodoquena, identify natural infections by leishmania spp. in insect vectors of cutaneous and visceral leishmaniasis, we performed field studies in natural and anthropic environments in the guaicurus settlement (bodoquena range) of the bonito municipality, mato grosso do sul state, brazil. from october 2002 to october 2003, a total of 1395 sandfly females were captured with shannon and light traps and dissected in search of flagellates. the sample is composed of a total of 13 species, with lutzomyia alme ...200919062193
[identification of leishmania species isolated in human cases in mato grosso do sul, by means of the polymerase chain reaction].leishmaniases are endemic zoonoses in the state of mato grosso do sul. their etiological agents in this region of brazil are leishmania (leishmania) chagasi, leishmania (leishmania) amazonensis and leishmania (viannia) braziliensis. the polymerase chain reaction (pcr) is a tool with high specificity and sensitivity for identifying leishmania species. this study examined 39 cryopreserved isolates of leishmania that had been collected by bone marrow aspiration and/or lesion biopsy, depending on th ...200919684979
phlebotomine fauna (diptera: psychodidae) of an american cutaneous leishmaniasis endemic area in the state of mato grosso do sul, brazil.the occurrence of an outbreak of cutaneous leishmaniasis associated with leishmania (leishmania) amazonensis in the municipality of bela vista, state of mato grosso do sul, brazil, and the absence of information on its vectors in this area led the authors to undertake captures of phlebotomine sand flies, using shannon traps and automatic cdc light traps, in domiciles, forested areas and animal shelters from february 2004-january 2006. a total of 808 specimens belonging to 18 sandfly species have ...200919820827
epidemiological study on leishmaniasis in an area of environmental tourism and ecotourism, state of mato grosso do sul, 2006-2007.the aims of this study were to carry out a serological survey of canine leishmaniasis and identify the phlebotomine fauna in the urban area of bonito, mato grosso do sul. the serological survey was conducted on a sample of 303 dogs, by means of the indirect immunofluorescence test. phlebotomines were captured using automated light traps. the serological survey found that 30% of the dogs were seropositive, both from the center and from all districts of the town. a total of 2,772 specimens of phle ...200919967228
[evidence of transmission of visceral leishmaniasis by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil].the municipality of jaciara was classified in 2003 as a transmission area for visceral leishmaniasis in outbreak situations. this study aimed to establish evidence of transmission of leishmania (leishmania) infantum chagasi by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil.201121340413
natural infection of leishmania (viannia) braziliensis in mus musculus captured in mato grosso, brazil.abstract we report natural infection by leishmania (viannia) braziliensis in mus musculus and necromys lasiurus using molecular analyses (pcr-rflp) of femoral bone marrow and skin fragments. the aim of this study was to detect infection by pathogenic species of leishmania in small mammals in the state of mato grosso, brazil. the animals were captured in peixoto de azevedo, a cutaneous leishmaniasis-endemic region located in the north of the state, from october 30 to november 3, 2008. natural i ...201121923255
nycthemeral rhythm of phlebotominae (diptera: psychodidae) in a craggy region, transitioning between the wetland and the plateau, brazil.recording the nycthemeral rhythm of sand flies allows the evaluation of the daily activity in different ecotypes, the period of greatest activity, and their degree of anthropophily. we investigated the fauna and the rhythm of sand fly activity in an ecotourism region in mato grosso do sul (ms) state, brazil. sand flies were captured monthly, using a shannon trap for 24 h periods between july 2012 and june 2014. we collected 1,815 sand flies, in which lutzomyia whitmani (=nyssomyia whitmani, sens ...201728082638
first record of leishmania braziliensis presence detected in bats, mato grosso do sul, southwest brazil.leishmaniasis, a zoonotic disease caused by parasites of the genus leishmania, has expanded beyond its natural range and is becoming increasingly urban. using pcr and pcr-rflp, we detected leishmania (viannia) braziliensis in two bats (chiroptera) in mato grosso do sul, brazil, an endemic area. this is the first record of l. (v.) braziliensis in bats. it is also the first record of any leishmania sp. in bats in the state. the animals testing positive were found in both a rural site and an urban ...201323886850
leishmania (v.) braziliensis infecting bats from pantanal wetland, brazil: first records for platyrrhinus lineatus and artibeus the new world genus leishmania parasites are etiological agents of neglected zoonoses known as leishmaniasis. its epidemiology is very complex due to the participation of several species of sand fly vectors and mammalian hosts, and man is an accidental host. control is very difficult because of the different epidemiological patterns of transmission observed. studies about leishmania spp. infection in bats are so scarce, which represents a large gap in knowledge about the role of these animals ...201728502644
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