montenegro skin tests for american cutaneous leishmaniasis carried out on school children in rio de janeiro, brazil: an indicator of transmission risk.montenegro skin tests were carried out in 1985 and 1987 on two groups of school children in the city of rio de janeiro. group a consisted of 449 children residing in the jacarepagua district, in areas where transmission of human and canine american cutaneous leishmaniasis (acl) has been high; this group was considered to be the one at greatest risk of acquiring the infection through l. braziliensis. group b consisted of 282 children from bonsucesso, a suburb of rio de janeiro that is at a consid ...19921363175
zoonotic cutaneous leishmaniasis due to leishmania (viannia) braziliensis associated with domestic animals in venezuela and brazil.after outbreaks of cutaneous leishmaniasis in solano state, venezuela, 5% of the population had parasitized ulcers while after similar outbreaks in mesquita, rio de janeiro state, brazil, 9% had the disease. in these foci children, including some under six years of age, were affected. there was no significant difference in the occurrence of the disease according to sex or type of employment. in solano, 3% of dogs and 28% of donkeys had parasitized lesions, while in mesquita these indices were 19 ...19892319948
indirect immunofluorescence test in new world leishmaniasis: serological and clinical relationship.the indirect immunofluorescence test (if) for anti-leishmania antibodies (igg and igm) was performed with sera from the following groups of individuals: 214 cutaneous leishmaniasis patients, 28 healthy subjects with positive montenegro's skin test (mst), 29 healthy subjects with negative mst and 16 visceral leishmaniasis patients. the first four groups came from a suburban area of rio de janeiro (jacarepaguá) where cutaneous leishmaniasis caused by leishmania braziliensis braziliensis is endemic ...19883078345
an outbreak of american cutaneous leishmaniasis (leishmania braziliensis braziliensis) in a periurban area of rio de janeiro city, brazil: clinical and epidemiological studies.from july 1984 to september 1986, 105 cases of american cutaneous leishmaniasis were studied in a locality closely situated to an urbanized area of the city of rio de janeiro, brazil. settlement in this area was established at least 20 years ago but the first cases were noted six months prior to the beginning of this study. cases were almost exclusively cutaneous and ulcerated, with one to six months of evolution. montenegro's skin tests were positive in all cases and anti-leishmania antibodies ...19883271941
canine american cutaneous leishmaniasis: a clinical and immunological study in dogs naturally infected with leishmania braziliensis braziliensis in an endemic area of rio de janeiro, brazil.clinical and immunological findings from 35 dogs infected with leishmania braziliensis braziliensis are described. the majority of the dogs had ulcerated single lesions on the ears. sera from all infected dogs showed detectable leishmania-induced antibodies using an indirect fluorescent antibody test. antimonial therapy resulted in prompt healing of the lesions in 80.9% of the animals followed by a significant reduction in the anti-leishmania antibody titers. however, treatment follow-up showed ...19883277465
human, canine and equine leishmaniasis caused by leishmania braziliensis braziliensis in an endemic area in the state of rio de janeiro. 19873507562
[phlebotomus of vargem grande, a focus of cutaneous leishmaniasis in the state of rio de janeiro].in vargem grande, an endemic area of cutaneous leishmaniasis in state of rio de janeiro, where lutzomyia intermedia had been found infected with leishmania braziliensis, we performed a series of captures of sandflies to increase the knowledge on their behaviour. the following species were found among 8,671 sandflies collected: l. intermedia, l. migonei, l. longipalpis, l. lanei, l. fischeri, l. firmatoi, l. monticola, l. schreiberi, l. whitmani, l. pelloni, l. barrettoi e l. (pi) sp. inside hous ...19863574131
identification and distribution of new world leishmania species characterized by serodeme analysis using monoclonal antibodies.five hundred thirty stocks of leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of american cutaneous (acl) and visceral leishmaniases (avl) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. this study confirms and extends our preliminary results on the high specificity of some of these ...19873826486
a survey for american cutaneous and visceral leishmaniasis among 1,342 dogs from areas in rio de janeiro (brazil) where the human diseases occur.there are areas in the periphery of rio de janeiro city where human cases of visceral and/or cutaneous leishmaniasis occur. the parasites have been identified as leishmania donovani and leishmania braziliensis braziliensis respectively. a survey for leishmaniasis was done among 1,342 dogs from those areas using an indirect immunofluorescent test. from the dogs, 616 came from areas where only human cases of visceral leishmaniasis occurred, 373 from an area where all human cases were of cutaneous ...19853910994
[natural infection of lutzomyia intermedia lutz & neiva, 1912, in an endemic area of visceral leishmaniasis of rio de janeiro].in a focus of cutaneous leishmaniasis in jacarepaguá, rio de janeiro, one specimen of lutzomyia intermedia was found naturally infected with leishmania braziliensis.19846535924
ecology of sandflies in a recent focus of cutaneous leishmaniasis in paraty, littoral of rio de janeiro state (diptera, psychodidae, phlebotominae). 19938107595
intralesional therapy of american cutaneous leishmaniasis with pentavalent antimony in rio de janeiro, brazil--an area of leishmania (v.) braziliensis transmission.the drug of choice for leishmaniasis is pentavalent antimony and different regimens are under continuous evaluation. the ideal therapy should be simple, effective, and with no or minor side-effects. in this paper we have studied the efficacy of intralesionally applied antimony in new world cutaneous leishmaniasis.19979248897
a low-dose antimony treatment in 159 patients with american cutaneous leishmaniasis: extensive follow-up studies (up to 10 years).the efficacy of an antimony regimen at the dose of 20 mg/kg/day for a 3-4-week period is well established in the treatment of american cutaneous leishmaniasis. several drug side effects, however, have been described and the search for more suitable regimens is advisable. in the present paper, the effect of a low dose (5 mg/kg/day for 30 days) of antimony was evaluated in 159 individuals from endemic regions of rio de janeiro state, brazil, an area of leishmania (v.) braziliensis transmission. pa ...19979430521
use of pcr in diagnosis of human american tegumentary leishmaniasis in rio de janeiro, brazil, the most common etiological agent of american tegumentary leishmaniasis is leishmania (viannia) braziliensis. in general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. the main purpose of the present work was to evaluate the pcr as a routine tool for the diagnosis of leishmaniasis. biopsy specimens from cutaneous or mucosal lesions were taken from 230 individuals from areas where leishmania is endemic: 216 patients who had ...199910325330
south american cutaneous leishmaniasis of the eyelids: report of five cases in rio de janeiro state, describe american cutaneous leishmaniasis of the eyelids and highlight the main clinical and diagnostic features of lesions, which are rare in this location.200010647737
american tegumentary leishmaniasis (atl) in rio de janeiro state, brazil: main clinical and epidemiologic de janeiro state in brazil is an endemic area of american tegumentary leishmaniasis (atl) induced by leishmania (viannia) braziliensis. objective our purpose was to describe the main clinical and epidemiologic characteristics of the disease in rio de janeiro state.200010940114
an interesting outbreak of leishmaniasis in a family in the state of rio de janeiro. 200111277964
ecology of american cutaneous leishmaniasis in the state of rio de janeiro, brazil.a study of the sand fly fauna was carried out in the endemic area ofamerican cutaneous leishmaniasis (acl) in the municipality of mesquita, state of rio de janeiro, in an area where dwellings (with humans and dogs infected by leishmania (viannia) braziliensis) have been recently visited by sloths. weekly captures were made during two consecutive years in two different sites (1 m and 600 m above sea level) and in three different habitats in each site (domestic, extra-domestic and sylvatic) using ...200212546456
american cutaneous leishmaniasis in two cats from rio de janeiro, brazil: first report of natural infection with leishmania (viannia) braziliensis.we describe the isolation of leishmania (viannia) braziliensis from two female cats with american cutaneous leishmaniasis in rio de janeiro, brazil. the isolates were identified as l. (v.) braziliensis by isoenzyme electrophoresis.200415024926
positive montenegro skin test among patients with sporotrichosis in rio de janeiro.we studied 52 patients with sporotrichosis confirmed by isolation of sporothrix schenckii and reactivity to the montenegro skin test (mst) during an ongoing outbreak of this mycosis in rio de janeiro. the objective was to emphasize the importance of parasitological confirmation and the possibility of incorrect diagnosis based on the lesion's appearance, epidemiological information, and immunological tests. the antigen used for the mst was conserved in either thimerosal 1:10,000 (group 1) or 0.4% ...200515589796
micro-geographical variation among male populations of the sandfly, lutzomyia (nyssomyia) intermedia, from an endemic area of american cutaneous leishmaniasis in the state of rio de janeiro, brazil.the genetic relationships among male lutzomyia (nyssomyia) intermedia (lutz & neiva) (diptera: psychodidae) from three populations from the same endemic area of american cutaneous leishmaniasis (acl) in the state of rio de janeiro, brazil, were compared. the sandflies were collected in three ecologically different habitats: domestic, extra-domestic and sylvatic over a total range of 800 m. three molecular markers were employed to assess population variation. based on mlee markers, it could not b ...200515752175
[prevalence of canine infection from endemic areas of american cutaneous leishmaniasis in paracambi district, rio de janeiro state, between 1992 and 1993].in the district of paracambi, state of rio de janeiro an epidemiological survey for american tegumentary leishmaniasis in canine population was carried out in endemic localities. a total of 179 dogs was registered and 138 (77.1%) examined for their clinical aspects, development of delayed hypersensitivity (dhs) with imunoleish(r) antigen and serological responses by indirect immunofluorescent reaction and enzyme-linked immunosorbent assay. in 9 (6.5%) dogs with active cutaneous lesions or suspec ...200515821792
identification of naturally infected lutzomyia intermedia and lutzomyia migonei with leishmania (viannia) braziliensis in rio de janeiro (brazil) revealed by a pcr multiplex non-isotopic hybridisation identify naturally infected lutzomyia spp. by leishmania (viannia) braziliensis, a pcr multiplex non-isotopic hybridisation assay was developed for the analysis of insect samples collected in distinct areas of the municipality of rio de janeiro (brazil), from march to december 2003. data from experimental infection indicate that the method can detect one individual infected insect out of ten. wild sand flies were classified and grouped into pools of 10 specimens each, reaching a total of 40 f ...200516143358
mixed infection with leishmania (viannia) braziliensis and leishmania (leishmania) chagasi in a naturally infected dog from rio de janeiro, brazil.we report here the first case of co-infection with leishmania (viannia) braziliensis and leishmania (leishmania) chagasi in a naturally infected dog from rio de janeiro, brazil. isoenzyme characterisation identified the parasites isolated in culture from the cutaneous lesion as l. (v.) braziliensis and the isolates from blood and lymph node as l. (l.) chagasi. pcr analysis using specific primers followed by molecular hybridisation for direct leishmania species identification in tissue fragments ...200616257024
post mortem parasitological evaluation of dogs seroreactive for leishmania from rio de janeiro, brazil.a parasitological study was conducted on 66 dogs seroreactive for leishmania captured as a control measure of visceral leishmaniasis in the state of rio de janeiro, brazil. biological samples from different anatomical sites were collected during autopsy of the animals and cultured on biphasic medium (nnn/schneider). the leishmania isolates were characterized by isoenzyme electrophoresis. leishmania was isolated from 80.3% of the animals: 12 animals with leishmania (viannia) braziliensis isolated ...200616520002
sporotrichosis: the main differential diagnosis with tegumentary leishmaniosis in dogs from rio de janeiro, brazil.seventy-four dogs from the state of rio de janeiro with ulcerated cutaneous lesions were submitted to clinical, dermatological, parasitological, mycological, histopathological and cytopathological exams, a leishmanin skin test, an indirect immunofluorescence (iif) test for leishmaniosis, and nonspecific laboratory tests such as blood count and serum biochemistry. sporothrix schenckii was isolated from 41 dogs and leishmania (viannia) braziliensis was isolated from 33 animals. most dogs with spor ...200716973286
clinical features of cutaneous and disseminated cutaneous leishmaniasis caused by leishmania (viannia) braziliensis in paraty, rio de janeiro.american tegumentary leishmaniasis (atl) caused by leishmania (viannia) braziliensis is endemic in rio de janeiro state (rj), where the disease shows epidemiologic and clinical characteristics distinct from those of atl in other brazilian regions. paraty is the second most important endemic area in rj; however, reports on leishmaniasis in this region refer to the occurrence of the disease without describing its characteristics.200818937655
leishmania (viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: evaluation by two molecular markers.analyses of mlee, rapd and lssp-pcr were used to compare the panel of american tegumentary leishmaniasis (atl) isolates obtained from lesions of patients with rare clinical manifestations of the disease and typical lesions. all of the 34 samples analyzed by mlee demonstrated similar electromorphic profiles with leishmania (viannia) braziliensis reference strain. through the rapd analysis, nine genetic profiles (genotypes) were identified. lssp-pcr corroborates the initial screening and phenetic ...200919135053
trypanosoma caninum n. sp. (protozoa: kinetoplastida) isolated from intact skin of a domestic dog ( canis familiaris) captured in rio de janeiro, unknown trypanosoma species was isolated from an axenic culture of intact skin from a domestic dog captured in rio de janeiro, brazil, which was co-infected with leishmania (viannia) braziliensis. giemsa-stained smears of cultures grown in different media revealed the presence of epimastigotes, trypomastigotes, spheromastigotes, transitional stages, and dividing forms (epimastigotes or spheromastigotes). the highest frequency of trypomastigotes was observed in rpmi (15.2%) and dmem (9.2%) med ...200919216827
[serological evaluation for detection of anti-leishmania antibodies in dogs and cats in the district of santa rita de cássia, municipality of barra mansa, state of rio de janeiro].from a canine case of american cutaneous leishmaniasis in the locality of santa rita de cássia, municipality of barra mansa, rio de janeiro, a serological survey was performed on 177 dogs and 43 cats. evaluation of the canine serum samples showed that 10% had a positive reaction in the indirect immunofluorescence test and 10.7% in the enzyme-linked immunosorbent assay. among the feline serum samples tested, none of the animals had a positive reaction in the indirect immunofluorescence test and o ...200919448930
immunoenzymatic assay for the diagnosis of american tegumentary leishmaniasis using soluble and membrane-enriched fractions from infectious leishmania (viannia) braziliensis.the diagnosis of american tegumentary leishmaniasis (atl) is based on the visualization or isolation of the parasite, which is a time-consuming and poorly sensitive method. in this study, we evaluated the accuracy and reliability of elisa for the diagnosis of atl using soluble (sf) and membrane-enriched (mf) antigen fractions obtained from an infectious strain of leishmania (viannia) braziliensis. a total of 152 serum samples investigated at a referral center in rio de janeiro, brazil, between 2 ...201020872561
laboratory tests performed on leishmania seroreactive dogs euthanized by the leishmaniasis control 2008, in the west zone of rio de janeiro municipality-brazil, the leishmaniasis control program identified 155 dogs with titers = 40 by indirect immunofluorescence (iif) on blood collected onto filter paper. the objective of this study was to describe the laboratory test findings performed in dogs euthanized by the leishmaniasis program control of rio de janeiro municipality. dogs were examined, subjected to euthanasia and collection of clinical specimens. parasite isolation was obtained in 2 ...201121349644
the sand fly fauna (psychodidae: phlebotominae) in the region of saquarema, state of rio de janeiro, brazil, an endemic area of cutaneous leishmaniasis transmission.cutaneous leishmaniasis, caused by leishmania (viannia) braziliensis, is sporadic in many rural and suburban areas of rio de janeiro state. an investigation was carried out during 2008/9 in the municipality of saquarema, rio de janeiro, southeast brazil, in order to identify the phlebotomine sand fly fauna. more than 2,100 sand flies were collected in peridomestic areas in two chicken coops using cdc light traps. nine species of phlebotomine sand flies were identified: nyssomyia intermedia, nyss ...201121366786
aspects of the ecology of phlebotomines (diptera: psychodidae: phlebotominae) in an area of cutaneous leishmaniasis occurrence, municipality of angra dos reis, coast of rio de janeiro state, brazil.over a complete two-year period, phlebotomine specimens were caught in an area of cutaneous leishmaniasis occurrence in the municipality of angra dos reis. a manual suction tube was used to catch phlebotomines on house walls, and also light traps in domestic and peridomestic settings and in the forest. this yielded 14,170 specimens of 13 species: two in the genus brumptomyia and eleven in the genus lutzomyia. l. intermedia predominantly in domestic and peridomestic settings, with little presence ...024626417
leishmaniasis transmission in an ecotourism area: potential vectors in ilha grande, rio de janeiro state, brazil.the south coast of rio de janeiro state, in brazil, is endemic for cutaneous and visceral leishmaniases and is frequently visited by tourists from different parts of the world. since the complex epidemiology of leishmaniases demands local studies, the goal of this study was to investigate the phlebotomine sand fly fauna and leishmaniases transmission in ilha grande, an ecotourism area of angra dos reis municipality.201324499568
first report of cutaneous leishmaniasis caused by leishmania (leishmania) infantum chagasi in an urban area of rio de janeiro, brazil.american tegumentary leishmaniasis (atl) is an infectious disease caused by protozoa of the genus leishmania, and transmitted by sandflies. in the state of rio de janeiro, almost all of the cases of american tegumentary leishmaniasis (atl) are caused by leishmania (viannia) braziliensis, while cases of visceral leishmaniasis (vl) are caused by leishmania (leishmania) infantum chagasi. the resurgence of autochthonous vl cases in rio de janeiro is related to the geographic expansion of the vector ...026603237
ecological aspects of phlebotomine sand flies (diptera, psychodidae, phlebotominae) in areas of american cutaneous leishmaniasis, in the municipality of paraty, rio de janeiro, brazil. i-index of abundance by location and type of capture.the description of the first and autochthonous case of diffuse cutaneous leishmaniasis in the municipality of paraty, state of rio de janeiro, brought the interest of this study. sand flies were captured over a 3-yr period. using manual suction tubes, sand flies were collected from the inner and outer walls of homes, in the living spaces of domestic animals, and in shannon light traps, which were set up outside homes and in the forest. cdc light traps were installed inside homes, around the exte ...201526336245
apoptosis and frequency of total and effector cd8+ t lymphocytes from cutaneous leishmaniasis patients during antimonial therapy.leishmaniasis is an important parasitic disease affecting millions worldwide. human cutaneous leishmaniasis (cl) is endemic in rio de janeiro, brazil, where is caused by leishmania braziliensis. the adaptive immune response is accountable for the healing of cl and despite of key role of cd8+ t cells in this immune response little is known about the cd8+ t lymphocytes frequencies, apoptosis and antigen-responsive cd8+ t lymphocytes of cl patients during antimonial therapy.201525870976
pcr associated with molecular hybridization detects leishmania (viannia) braziliensis in healthy skin in canine tegumentary leishmaniasis.tegumentary leishmaniasis (tl) is a zoonotic disease that affects humans and domestic dogs. in brazil, tl is considered endemic, and leishmania (viannia) braziliensis is the prevalent species causing this disease. there is debate about the role of dogs (canis familiaris) as domestic reservoirs in the transmission cycle of tl. to date, classical parasitological techniques, including parasite isolation in culture media, have been able to detect parasites only from cutaneous lesions. in this study, ...201525158080
cutaneous and visceral leishmaniasis co-infection in dogs from rio de janeiro, brazil: evaluation by specific pcr and rflp-pcr assays.during a diagnostic evaluation of canine visceral leishmaniasis (vl), two of seventeen dogs were found to be co-infected by leishmania (viannia) braziliensis and leishmania (leishmania) chagasi.201424861303
canine cutaneous leishmaniasis caused by neotropical leishmania infantum despite of systemic disease: a case report.visceral leishmaniasis is an anthropozoonosis caused by a protozoan leishmania infantum (syn. leishmania chagasi). here, we report a typical case of canine cutaneous leishmaniasis due to l. infantum infection without any other systemic symptom in one dog in the city of rio de janeiro, brazil. a mongrel female dog was admitted in a veterinary clinic with reports of chronic wounds in the body. physical examination revealed erosive lesions in the limbs, nasal ulcers, presence of ectoparasites and s ...201222583758
trichophoromyia auraensis is a putative vectorcomments about the finding of trichophoromyia auraensis (mangabeira 1942) sandfly specimens infected by leishmania (viannia) parasites in acre statea new sandfly in the subgenus nyssomyia (diptera, psychodidae) from the amazon basin of brazilthe known geographical distribution of sandflies in the state of acre, brazil (diptera: psychodidae)studies on the sandfly fauna (diptera: psychodidae: phlebotominae) from transmission areas of american cutaneous leishmaniasis in state of acre, brazildevelopment and validation of pcr-based assays for diagnosis of american cutaneous leishmaniasis and identification of the parasite speciesfirst description of leishmania (viannia) infection in evandromyia saulensis, pressatia sp. and trichophoromyia auraensis (psychodidae: phlebotominae) in a transmission area of cutaneous leishmaniasis in acre state, amazon basin, brazilsand fly fauna (diptera: psychodidae: phlebotominae) in an area of leishmaniasis transmission in the municipality of rio branco, state of acre, brazilidentification of naturally infected lutzomyia intermedia and lutzomyia migonei with leishmania (viannia) braziliensis in rio de janeiro (brazil) revealed by a pcr multiplex non-isotopic hybridization assaynotas sobre os flebotomíneos do estado do acre, com a descrição de duas espécies novas (diptera, psychodidae)description of trichophoromyia ruifreitasi, a new phlebotomine species (diptera, psychodidae) from acre state, brazilian amazonepidemiologia da leishmaniose tegumentar e descrição das populações de flebotomíneos no município de acrelândia, acre, brasilphlebotomine sandfly (diptera: psychodidae) diversity and their leishmania dna in a hot spot of american cutaneous leishmaniasis human cases along the brazilian border with peru and boliviadescription of a new phlebotomine species (diptera: psychodidae, phlebotominae) and new records of sand flies from the state of acre, northern brazilnatural leishmania infection of lutzomyia (trichophoromyia) auraensis in madre de dios, peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reactionguide to the identification and geographic distribution of lutzomyia sandflies in mexico, the west indies, central and south america (diptera: psychodidae).the sandfly trichophoromyia auraensis has recently evolved as a proven vector of leishmania (viannia) endemic to state of acre in the north of brazil. this note is intended to propose a correction in the report of the first occurrence of natural infection of leishmania (viannia) in this species. we and the other scientific groups reinforced that tr. auraensis is a possible vector involved in the transmission of american cutaneous leishmaniasis in acre, brazil.201728591315
sporotrichoid leishmaniasis: a cross-sectional clinical, epidemiological and laboratory study in rio de janeiro state, brazil.atypical presentations of cutaneous leishmaniasis include sporotrichoid leishmaniasis (sl), which is clinically described as a primary ulcer combined with lymphangitis and nodules and/or ulcerated lesions along its pathway.201728591261
low versus high dose of antimony for american cutaneous leishmaniasis: a randomized controlled blind non-inferiority trial in rio de janeiro, brazil.although high dose of antimony is the mainstay for treatment of american cutaneous leishmaniasis (acl), ongoing major concerns remain over its toxicity. whether or not low dose antimony regimens provide non-inferior effectiveness and lower toxicity has long been a question of dispute.201728558061
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