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contribution to the sand fly fauna (diptera: phlebotominae) of rio grande do sul, brazil and leishmania (viannia) infections.american cutaneous leishmaniasis (acl) is endemic in the state of rio grande do sul, brazil. as reports of nearby hospitals suggest, the parque estadual do turvo seems to serve as a source for the disease. during three months from november 1996 to february 1997 we collected, in this park, 2,228 sandflies (10 lutzomyia species and 2 6species). we applied the polymerase chain reaction to 920 females which belong to the following species: lutzomyia migonei, lu. pessoai, lu. fischeri, lu. misionensi ...199910464396
first report of lutzomyia (nyssomyia) neivai (diptera: psychodidae: phlebotominae) naturally infected by leishmania (viannia) braziliensis in a periurban area of south brazil using a multiplex polymerase chain reaction assay.to identify lutzomyia (nyssomyia) neivai naturally infected by leishmania a multiplex polymerase chain reaction (pcr) was used for the analysis of 450 specimens (270 females, 180 males) collected in an endemic periurban area of cutaneous leishmaniasis in porto alegre, brazil. insects were grouped into pools of 10 and positive results were achieved in 3/27 lu. (n.) neivai female pools. infection by l. (viannia) braziliensis was confirmed after hybridizing pcr products with a subgenus-specific bio ...200919346382
lutzomyia (pintomyia) fischeri (diptera: psychodidae: phlebotominae), a probable vector of american cutaneous leishmaniasis: detection of natural infection by leishmania (viannia) dna in specimens from the municipality of porto alegre (rs), brazil, using multiplex pcr assay.in order to determine natural leishmania (viannia) infection in lutzomyia (pintomyia) fischeri, a multiplex pcr methodology coupled to non-isotopic hybridization was adopted for the analysis of sand fly samples collected by cdc light traps in an endemic area of american cutaneous leishmaniasis (acl) in the periurban region of the municipality of porto alegre, rio grande do sul state, brazil. we analyzed by pcr methodology 560 specimens of lutzomyia (pintomyia) fischeri (520 females and 40 males) ...201121939631
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