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human cutaneous leishmaniasis due to a new enzymatic variant of leishmania (viannia) braziliensis occurring in pernambuco, brazil. 19938139470
morphometric and phenetic studies of five geographical populations of lutzomyia whitmani (diptera: psychodidae) in brazil.a morphometric survey examined adult specimens of lutzomyia whitmani (antunes & coutinho) captured at 5 municipalities in southeastern and northeastern brazil to compare the populations. the localities were ilhéus (bahia), martinho campos (minas gerais), corte de pedra (bahia), baturité (ceará), and amaraji (pernambuco): all are known foci of american cutaneous leishmaniasis. fifteen males and 15 females from each population were analyzed morphometrically for 42 and 37 characters, respectively. ...199910593089
epidemiological surveys confirm an increasing burden of cutaneous leishmaniasis in north-east brazil.health service records for north-east brazil suggest a consistent rise in numbers of cases of cutaneous leishmaniasis due to leishmania (viannia) braziliensis over the past decade. in a study site in pernambuco, prospective, cross-sectional and retrospective epidemiological surveys of infection (a positive montenegro skin test response) and/or clinical symptoms confirmed a high current force of infection (0.092/year), and an approximately 10-fold increase in transmission during the last 10 years ...199910696402
evaluation of pcr for diagnosis of american cutaneous leishmaniasis in an area of endemicity in northeastern brazil.pcr-based approaches targeting kinetoplast dna were evaluated for the diagnosis of american cutaneous leishmaniasis (acl) in regions of endemicity in northeastern brazil. a total of 119 cutaneous biopsy specimens from patients with acl and nonleishmaniasis cutaneous lesions were studied. two pcr-based systems were used; one was specific for the subgenus viannia, and the other was specific for the genus leishmania. the pcr specific for the subgenus viannia had a sensitivity of 95.4%, whereas the ...200212354848
wild and synanthropic hosts of leishmania (viannia) braziliensis in the endemic cutaneous leishmaniasis locality of amaraji, pernambuco state, brazil.evidence of leishmania infection was found in small mammals captured between 1996 and 2000 in the amaraji region, pernambuco state, brazil. the kdna polymerase chain reaction (pcr), using primers specific for subgenus l. (viannia), was positive for 43/153 water rats (nectomys squamipes), 13/81 black rats (rattus rattus), 15/103 grass mice (bolomys lasiurus), 1/14 marsh mice (holochilus scieurus), 2/50 field mice (akodon arviculoides), 2/12 woolly opossums (marmosa sp.), and 5/37 common opossums ...200315228244
[american tegumentary leishmaniasis caused by leishmania (viannia) braziliensis in military training area of zona da mata in pernambuco].the aim of work was to study the epidemiology of american tegumentary leishmaniasis in a military training unit situated in zona da mata region of pernambuco state. between 2002 and 2003 twenty-three cases were notified by clinical exam, detection and/or isolation of parasite and montenegro skin test. seven stocks of leishmania (viannia) braziliensis were obtained from patients, identified by a panel of specific monoclonal antibodies and isoenzymatic electrophoresis profiles. an epidemiologic su ...200515895173
sandfly fauna in a military training area endemic for american tegumentary leishmaniasis in the atlantic rain forest region of pernambuco, brazil.the aim of this study was to characterize the sandfly fauna in a military training area situated in the "zona da mata" region of pernambuco state, brazil, where human cases of american tegumentary leishmaniasis (atl) had been observed, caused by leishmania (viannia) braziliensis. the occurrence of 16 sandfly species in the marshall newton cavalcanti military training camp (cimnc) indicates an important diversity of sandfly species in this study area. lutzomyia complexa was the most common specie ...200616410860
clinical, epidemiological and laboratory aspects of patients with american cutaneous leishmaniasis in the state of pernambuco.the diagnosis for american cutaneous leishmaniasis is based on an association of clinical, epidemiological and laboratory characteristics. the present study identified the circulating species of leishmania in the state of pernambuco, described its clinical-epidemiological characteristics and diagnosed the disease. nineteen patients presenting active lesions who had been diagnosed through clinical evaluation and laboratory tests were selected. the tests included direct investigation, in vitro cul ...200819009182
[new outbreak of american tegumentary leishmaniasis in a military training center in the zona da mata region, in the north of the state of pernambuco].a new outbreak of american tegumentary leishmaniasis among military personnel is reported, with 71 cases confirmed by means of clinical, epidemiological and laboratory criteria. seven samples were isolated and were identified as leishmania (viannia) braziliensis. the occurrence of outbreaks in this region confirms the endemic nature of this disease, and the magnitude of the occurrence seems to be related to non-adoption of individual protection measures.200919967247
detection of leishmania braziliensis dna in american tegumentary leishmaniasis patients.diagnostic tests for american tegumentary leishmaniasis were performed on blood samples of patients living in two endemic municipalities in the state of pernambuco, northeastern brazil. dna was extracted from 119 samples and used as template for polymerase chain reaction (pcr) analysis. the tests used primers specific for the kinetoplast mini-circle dna (kdna) of leishmania braziliensis, a species circulating in pernambuco, which amplify a 750 base pair target sequence. in total, 58 subjects (48 ...201020428600
infectiousness of sylvatic and synanthropic small rodents implicates a multi-host reservoir of leishmania (viannia) braziliensis.the possibility that a multi-host wildlife reservoir is responsible for maintaining transmission of leishmania (viannia) braziliensis causing human cutaneous and mucocutaneous leishmaniasis is tested by comparative analysis of infection progression and infectiousness to sandflies in rodent host species previously shown to have high natural infection prevalences in both sylvatic or/and peridomestic habitats in close proximity to humans in northeast brazil.201526448187
cutaneous leishmaniasis in northeastern brazil: a critical appraisal of studies conducted in state of pernambuco.american cutaneous leishmaniasis (acl) is a complex disease with clinical and epidemiological features that may vary from region to region. in fact, at least seven different leishmania species, including leishmania (viannia) braziliensis, leishmania (viannia) guyanensis, leishmania (viannia) lainsoni, leishmania (viannia) naiffi, leishmania (viannia) shawi, leishmania (viannia) lindenbergi, and leishmania (leishmania) amazonensis, have been implicated in the etiology of acl in brazil, and numero ...201222836662
clinical and hematological findings in leishmania braziliensis-infected dogs from pernambuco, brazil.canine cutaneous leishmaniasis by leishmania braziliensis is a neglected, but widespread disease of dogs in south america. this paper describes clinical and hematological alterations in 17 l. braziliensis-infected dogs from brazil. the most common hematological findings were thrombocytopenia (82.4%), anemia (70.6%), low packed cell volume (52.9%) and eosinophilia (41.2%). twelve (70.6%) dogs displayed at least one evident physical alteration; 11 dogs (64.7%) presented skin lesions, four (23.5%) ...201323207982
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