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the survey of porcine teschoviruses, porcine circovirus and porcine transmissible gastroenteritis virus infecting piglets in clinical specimens in china.a multiplex pcr assay was developed and evaluated for its ability to simultaneously detect three viral infections of swine. specific primers were carefully selected from articles published for each of the following three viruses: porcine circovirus type ii (pcv2), porcine teschovirus (ptv) and porcine transmissible gastroenteritis virus (tgev). each target produced a specific amplicon with a size of 353 bp (pcv2), 168 bp (ptv) and 499 bp (tgev). the sensitivity of the multiplex pcr using purifie ...201323224864
isolation and characterization of the first chinese strain of porcine teschovirus-8.investigations were carried out to identify the causal agent of acute diarrhea, respiratory distress, and death of pigs on a swine farm in jilin province, northern china. only porcine teschovirus (ptv, designated as ptv-8 jilin/2003) was isolated from samples of organs. the presence of ptv was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results of the immunoperoxidase monolayer assay (ipma), polymerase chain reaction, and electron microscopy. other ...201020362007
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