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isolation of st. louis encephalitis virus from overwintering culex pipiens mosquitoes.two strains of st. louis encephalitis virus were isolated from overwintering mosquitoes collected in maryland and pennsylvania during january and february 1977. there isolations from culex pipiens constitute evidence that a mosquito-borne flavivirus can persist in a vector mosquito in temperate climates during the winter season.1978628843
use of base excision sequence scanning for detection of genetic variations in st. louis encephalitis virus isolates.twenty-two isolates of st. louis encephalitis (sle) virus of various geographical origins (brazil, argentina, panama, texas, missouri, maryland, california, and florida) were examined for genetic variation by the base excision sequence scanning (bess t-scan) method. a fragment was amplified in the envelope gene with the forward primer labeled in the pcr. the bess t-scan method determined different clusters according to the profiles generated for the isolates and successfully grouped the isolates ...199910325350
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