| using msa-2b as a molecular marker for genotyping mexican isolates of babesia bovis. | variable merozoite surface antigens of babesia bovis are exposed glycoproteins having a role in erythrocyte invasion. members of this gene family include msa-1 and msa-2 (msa-2c, msa-2a(1), msa-2a(2) and msa-2b). to determine the sequence variation among b. bovis mexican isolates using msa-2b as a genetic marker, pcr amplicons corresponding to msa-2b were cloned and plasmids carrying the corresponding inserts were purified and sequenced. comparative analysis of nucleotide and deduced amino acid ... | 2009 | 19931189 |
| bovine babesiosis and anaplasmosis follow-up on cattle relocated in an endemic area for hemoparasitic diseases. | a multiplex pcr/dna probe assay was used to monitor babesia bovis, b. bigemina and anaplasma marginale infection in cattle introduced to a boophilus microplus-infested area in veracruz, mexico. eight intact, 18-month-old, cross-bred beef cattle (four naive, group a; four babesia species--premunized, group b) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (pe) to ticks. blood sample analysis for dna detection by the mpcr/dna probe ... | 1998 | 9668444 |