| antigenic profile of a pure isolate of anaplasma marginale of brazilian origin, using a western blot technique. | anaplasma marginale initial bodies of the brazilian isolate aufv1 were purified from infected erythrocytes using a combination of lysis, ultrasonic disruption and differential centrifugation. initial bodies were solubilised with a buffer containing protease inhibitors and non-ionic detergents. immunochemical analysis by the western blot technique revealed at least five proteins with apparent molecular weights (mw) of 105, 100, 97, 87 and 38 kda when homologous sera were used as primary antibodie ... | 1994 | 8030178 |
| molecular detection and identification of hemoparasites in pampas deer (ozotoceros bezoarticus linnaeus, 1758) from the pantanal brazil. | hemoparasites were surveyed in 60 free-living pampas deer ozotoceros bezoarticus from the central area of the pantanal, known as nhecolândia, state of mato grosso do sul, brazil, through the analysis of nested pcr assays and nucleotide sequencing. blood samples were tested for babesia/theileria, anaplasma spp., and trypanosoma spp. using npcr assays and sequencing of the 18s rrna, msp4, its, and cathepsin l genes. the identity of each sequence was confirmed by comparison with sequences from genb ... | 2013 | 23567028 |