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isolation and characterization of epizootic hemorrhagic disease virus from white-tailed deer (odocoileus virginianus) in eastern washington.a virus was isolated from the spleen of a white-tailed deer (odocoileus virginianus) that had died during an epizootic in washington state in 1967. inoculation of a 10% spleen suspension from the deer caused hemorrhagic disease in normal white-tailed deer. studies were conducted on the biological, physicochemical, and serologic properties of the washington isolate. an in vitro assay system, utilizing a cultured primary of white-tailed deer fetal cells from an entire fetus, was employed for isola ...1977192110
geographical genetic variation in the gene encoding vp3 from the alberta isolate of epizootic hemorrhagic disease virus.the complete nucleic acid and deduced amino acid sequences of gene segment 3 and the encoded vp3 from the north american, alberta isolate of epizootic hemorrhagic disease virus serotype 2 (ehdv-2) are reported. complementary dna corresponding to segment 3 was 2768 nucleotides in length with an open reading frame of 2697 base pairs which encoded a vp3 polypeptide of 899 amino acid residues. sequence comparison with genome segment 3 and vp3 from the australian strain of ehdv-2 indicated genotypic ...19957653105
development of polymerase chain reaction for specific identification of epizootic hemorrhagic disease virus serotype 1.the diagnostic potential of the polymerase chain reaction (pcr) for specific identification of epizootic hemorrhagic disease virus serotype 1 (ehdv-1) in cell culture and clinical specimens was evaluated. using oligonucleotide primers, selected from genome segment 2 of ehdv-1 (new jersey strain), the pcr-based assay resulted in a 862 base pair (bp) pcr product. ehdv-1 rna from united states prototype serotype 1 and a number of ehdv-1 field isolates, propagated in cell cultures, were detected by ...19958572948
molecular characterization of epizootic hemorrhagic disease virus serotype 1 associated with a 1999 epizootic in white-tailed deer in the eastern united states.during the autumn of 1999 (mid-august-late september), an outbreak of hemorrhagic disease in white-tailed deer (odocoileus virginianus) caused by epizootic hemorrhagic disease virus serotype 1 (ehdv-1) occurred along the east coast of the united states from georgia to new jersey. an ehdv-1 epizootic of such magnitude had not been described in this region since 1975. to determine the genetic relatedness among the 1999 viruses, as well as among additional ehdv-1 isolates from the eastern and weste ...200617092892
detection of bluetongue virus rna in field-collected culicoides spp. (diptera: ceratopogonidae) following the discovery of bluetongue virus serotype 1 in white-tailed deer and cattle in louisiana.in november 2004, bluetongue virus (family reoviridae, genus orbivirus, btv) serotype 1 (btv-1) was detected for the first time in the united states from a hunter-killed deer in st. mary parish, la. in 2005, sera surveys were conducted on three cattle farms near the area where the deer was found, and btv-1-seropositive cattle were found on two of the three farms; in 2006, sera surveys from the cattle on the three farms did not detect any btv-1-positive animals. the purpose of this study was to s ...201020380309
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