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baculovirus expression of turkey coronavirus nucleocapsid protein.the nucleocapsid (n) gene of turkey coronavirus (tcv) was amplified by reverse transcriptase-polymerase chain reaction, cloned, and expressed in the baculovirus expression system. a recombinant baculovirus containing the tcv n gene (rbtcv/n) was identified by polymerase chain reaction and expression of tcv n protein as determined by western immunoblot analysis. two tcv-specific proteins, 52 and 43 kda, were expressed by rbtcv/n; one of these proteins, p52, was comparable in size to native tcv n ...200111332474
comparison of 3'-end encoding regions of turkey coronavirus isolates from indiana, north carolina, and minnesota with chicken infectious bronchitis coronavirus strains.to analyze the 3'-end structural protein-encoding region of turkey coronavirus (tcov) isolates associated with outbreaks of acute enteritis in indiana, north carolina, or minnesota.200616491018
antigenic relationship of turkey coronavirus isolates from different geographic locations in the united states.the purpose of the present study was to examine the antigenicity of turkey coronavirus (tcv) isolates from various geographic areas with antibodies to different viruses. seventeen isolates of tcv were recovered from intestinal samples submitted to animal disease diagnostic laboratory, purdue university, from turkey farms located in different geographic areas. the prototype tcv minnesota isolate (tcv-atcc) was obtained from the american type culture collection. intestinal sections were prepared f ...200212061660
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