| genotyping of newly isolated infectious bronchitis virus isolates from northeastern georgia. | sixteen infectious bronchitis virus (ibv) field isolates obtained from vaccinated commercial broiler chickens showing clinical respiratory disease were characterized by reverse transcriptase-polymerase chain reaction and sequence analysis of the hypervariable region of the s1 spike glycoprotein gene. the genetic relationship among these variants and reference strains was determined by phylogenetic analysis and use of the basic local alignment search tool. all the isolates formed a distinct phylo ... | 2010 | 21313832 |
| data from 11 years of molecular typing infectious bronchitis virus field isolates. | in 1993, a new molecular typing method for infectious bronchitis virus (ibv) was introduced. this method uses reverse transcriptase-polymerase chain reaction (rt-pcr) and restriction fragment length polymorphism (rflp) analysis of the spike gene to obtain rflp patterns that correlate with serotype. using that test at the poultry diagnostic and research center (pdrc, university of georgia, athens, ga), we have identified a total of 1523 ibv isolates in the past 11 yr. the data were obtained from ... | 2005 | 16405010 |
| genetic relationships of infectious bronchitis virus isolates from mississippi broilers. | a 582-base pair segment located in the nucleocapsid protein terminal part of the s1 gene of 26 arkansas (ark)-type infectious bronchitis virus (ibv) isolates from mississippi broilers was amplified and sequenced. reverse transcription-polymerase chain reaction and cycle sequencing techniques were used to elucidate the genetic and deduced amino acid relationships among the isolates. analysis suggested that the nucleotide and amino acid sequences of the isolates were highly conserved, with greater ... | 2000 | 10737646 |
| sequence comparison of avian infectious bronchitis virus s1 glycoproteins of the florida serotype and five variant isolates from georgia and california. | the infectious bronchitis virus (ibv) spike glycoprotein s1 subunit is required to initiate infection and contains virus-neutralizing and serotype-specific epitope(s). reported are the s1 gene nucleotide and predicted amino acid sequences for the florida 18288 strain and isolates ga-92, cv-56b, cv-9437, cv-1686, and 1013. these sequences were compared with previously published gene sequences of ibv strains, and phylogenetic relationships are reported. the s1 amino acid sequence of florida 18288 ... | 1998 | 9778790 |
| isolation of georgia variant (georgia isolate 1992) infectious bronchitis virus but not ornithobacterium rhinotracheale from a kentucky broiler complex. | integrated broiler production operations in western kentucky have been very successful. the reason for this success includes the fact that flocks are free of many endemic diseases for a period of time, often years, because birds are raised in virgin, disease-free territory. this case report documents that importation of birds from an area with endemic georgia variant (ga-92) infectious bronchitis virus and ornithobacterium rhinotracheale (ort) bacterial infection resulted in introduction of ga-9 ... | 1998 | 9777165 |
| protection by a commercial arkansas-type infectious bronchitis virus vaccine against a field isolate of the same serotype. | a late-breaking infectious bronchitis virus (ibv)-associated respiratory disease was a chronic problem in georgia broilers in 1995. the predominant virus isolated from diseased birds was the arkansas (ark) type of ibv. because broilers in georgia are currently vaccinated with the arkansas serotype, there was concern that a phenotypic and/or genotypic change had occurred in the field virus so it could break through immunity conferred by commercial vaccines. the purpose of this study was to determ ... | 1997 | 9454933 |
| in vivo evaluation of the pathogenicity of field isolates of infectious bronchitis virus. | the pathogenicity of 13 field isolates of infectious bronchitis virus (ibv) isolated from georgia broiler farms from 1989 to 1992 was evaluated using the ibv and escherichia coli mixed-infection model. based on the clinical signs, mortality, and lesions, the isolates were classified as high, intermediate, and low in pathogenicity. the in vivo classification was compared with the serotype classification results obtained by reverse transcriptase-polymerase chain reaction-restriction fragment lengt ... | 1994 | 7832713 |
| emergence of subtype strains of the arkansas serotype of infectious bronchitis virus in delmarva broiler chickens. | infectious bronchitis virus (ibv) field isolates of the arkansas (ark) serotype were identified by reverse transcription-polymerase chain reaction (rt-pcr) as the most common serotype isolated from 1993 to 1997. these isolates were recovered from broiler flocks with respiratory disease raised on the delmarva peninsula in spite of ark vaccination in the region. for the purposes of investigating this apparently paradoxical finding, five rt-pcr ark-positive field isolates recovered in 1995 and 1996 ... | 2000 | 11007004 |
| identification and analysis of the georgia 98 serotype, a new serotype of infectious bronchitis virus. | twenty-five field infectious bronchitis viruses (ibvs) similar to, but genetically distinct from, the de072 serotype were isolated from several states in the united states from 1990 through 1999 and were examined molecularly and antigenically. a 421-bp sequence in the hypervariable region of the s1 gene was examined, and phylogenetic analysis on that region indicated that these viruses are closely related but fall into unique groups. cross-virus neutralization testing and entire s1 sequence anal ... | 2001 | 11332478 |
| simultaneous detection of five major serotypes of avian coronavirus by a multiplex microsphere-based assay. | avian coronavirus (commonly known as infectious bronchitis virus [ibv]) is of major economic importance to commercial chicken producers worldwide. due to the existence of multiple serotypes and variants of the virus that do not cross-protect, it is important to diagnose circulating serotypes and choose the right vaccine type for successful protection. in an effort to improve conventional diagnostic tests, a microsphere-based assay was developed and evaluated for simultaneous detection of the mos ... | 2013 | 23847226 |