| genomic analysis using pulsed-field gel electrophoresis of escherichia coli o157: h7 isolated from dairy calves during the united state national dairy heifer evaluation project (1992-1992). | the genomic fingerprints of 26 escherichia coli o157:h7 isolates from calves on 20 farms in 16 states were determined by pulsed-field gel electrophoresis (pfge). digestion of genomic dnas with the restriction enzymes sfii and xbai yielded 14 and 18 restriction endonuclease digestion profiles (redp), respectively. seventeen farms (85%) had e. coli o157:h7 with a unique redp, and when more than one calf tested positive on a farm, the isolates displayed identical redp. isolates from different farms ... | 1996 | 9054119 |
| clonal dissemination of escherichia coli o157:h7 subtypes among dairy farms in northeast ohio. | to ascertain the extent to which indistinguishable strains of escherichia coli o157:h7 are shared between farms, molecular characterization was performed on e. coli o157:h7 isolates recovered during a longitudinal study of 20 dairy farms in northeast ohio. of the 20 dairy farms sampled, 16 were located in a primary area and 4 were located in two other distant geographical areas. a total of 92 e. coli o157:h7 isolates obtained from bovine fecal samples, water trough sediment samples, free-stall b ... | 2006 | 16597966 |
| salmonella enterica isolated from wildlife at two ohio rehabilitation centers. | between may and september 2004, fecal samples from various wildlife species admitted to two rehabilitation centers in ohio were cultured for salmonella enterica and escherichia coli o157:h7. eight of 71 (11%) samples, including specimens from three opossums (didelphis virginiana), two gray squirrels (sciurus carolinensis), a woodchuck (marmota monax), a harris hawk (parabuteo unicinctus), and a screech owl (otus asio) tested positive for salmonella serovars braenderup, senftenberg, oranienburg, ... | 2007 | 17939349 |
| assessment of diagnostic tools for identifying cattle shedding and super-shedding escherichia coli o157:h7 in a longitudinal study of naturally infected feedlot steers in ohio. | the objectives of this study were to compare the performance of different diagnostic protocols (rectoanal mucosal swabs and immunomagnetic separation [rams-ims], fecal samples and ims [fecal-ims], and direct plating) to determine the prevalence of escherichia coli o157:h7 and to evaluate the pattern of e. coli o157:h7 shedding and super-shedding (defined as having a direct plating count equal to or >10(4) colony forming units of e. coli o157:h7 per gram of feces) in a longitudinal study of natur ... | 2010 | 21034264 |
| low prevalence of escherichia coli o157:h7 in horses in ohio, usa. | manure from draft animals deposited in fields during vegetable and fruit production may serve as a potential source of preharvest pathogen contamination of foods. to better quantify this risk, we determined the prevalence of escherichia coli o157:h7 in horses. between june and september 2009, freshly voided fecal samples were collected from horses stabled on 242 separate premises in ohio, usa. overall, the prevalence of e. coli o157:h7 was 1 of 242 (0.4% prevalence, 95% confidence interval [ci] ... | 2010 | 21219723 |
| point-counterpoint: should all stools be screened for shiga-toxin producing escherichia coli (stec)? | in october 2009, the centers for disease control and prevention recommended that clinical laboratories test all stools submitted for the detection of enteric bacterial pathogens for the presence of stec. in order to do this, it is recommended that all stools be cultured for escherichia coli o157:h7 on selective medium as well as testing for the presence of shiga toxin by immunoassay to detect non-o157 stec (3). there are a variety of products that are fda approved for detection of shiga toxin. f ... | 2011 | 21543568 |
| multiple-locus variable-nucleotide tandem repeat subtype analysis implicates european starlings as biological vectors for escherichia coli o157:h7 in ohio, usa. | aims: to provide molecular epidemiological evidence of avian transmission of escherichia coli o157:h7 between dairy farms in ohio, this study was designed to identify genetic relatedness between isolates originating from bovine faecal samples and intestinal contents of european starlings captured on these farms. methods and results: during a three-year period (2007-2009), cattle (n = 9000) and starlings (n = 430) on 150 different dairy farms in northern ohio were sampled for the presence of e. ... | 2011 | 21762472 |
| microbial and chemical contamination during and after flooding in the ohio river-kentucky, 2011. | surface water contaminants in kentucky during and after 2011 flooding were characterized. surface water samples were collected during flood stage (may 2-4, 2011; n = 15) and after (july 25-26, 2011; n = 8) from four different cities along the ohio river and were analyzed for the presence of microbial indicators, pathogens, metals, and chemical contaminants. contaminant concentrations during and after flooding were compared using linear and logistic regression. surface water samples collected dur ... | 2014 | 24967556 |
| microbial quality and bacteria pathogens in private wells used for drinking water in northeastern ohio. | in agricultural intensive areas, drinking contaminated water from private wells is considered an important cause of acute gastroenteric illnesses (agi), particularly among high-risk populations. in the summer of 2009, the microbial water quality of 180 randomly selected private wells in two northeastern ohio counties, a region with a high concentration of dairy farms, was assessed. forty-five percent (82/180) of water samples were contaminated with total coliforms. generic escherichia coli were ... | 2013 | 23981882 |
| a longitudinal study of feed contamination by european starling excreta in ohio dairy farms (2007-2008). | the objectives of this study were to understand the temporal pattern of contamination of cattle feed by starling excrement on dairy farms and to evaluate the temporal pattern in recovering escherichia coli o157:h7 or salmonella in relation to the absolute mass of excrement recovered. a longitudinal study was conducted on 15 dairy farms in ohio from july 2007 to october 2008. one open-topped tray filled with bird feed was placed near a cattle feeding site; bird excrement from the tray was weighed ... | 2014 | 24881798 |
| spatial epidemiology of escherichia coli o157:h7 in dairy cattle in relation to night roosts of sturnus vulgaris (european starling) in ohio, usa (2007-2009). | the goal of our study was to use spatial scan statics to determine whether the night roosts of european starlings (sturnus vulgaris) act as point sources for the dissemination of escherichia coli o157:h7 among dairy farms. from 2007 to 2009, we collected bovine faecal samples (n = 9000) and starling gastrointestinal contents (n = 430) from 150 dairy farms in northeastern ohio, usa. isolates of e. coli o157:h7 recovered from these samples were subtyped using multilocus variable-number tandem repe ... | 2014 | 24279810 |
| association of wild bird density and farm management factors with the prevalence of e. coli o157 in dairy herds in ohio (2007-2009). | our objective was to determine the role that european starlings (sturnus vulgaris) play in the epidemiology of escherichia coli o157:h7 in dairy cattle. we visited 150 dairy farms in ohio twice during summer and fall months from 2007 to 2009. fresh faecal pats from 30 lactating cows were collected during each visit. information on farm management and environmental variables was gathered through a questionnaire administered to the farm owner. the number of starlings observed on the farm was also ... | 2012 | 22333436 |
| soil conditions that can alter natural suppression of escherichia coli o157:h7 in ohio specialty crop soils. | food-borne pathogen persistence in soil fundamentally affects the production of safe vegetables and small fruits. interventions that reduce pathogen survival in soil would have positive impacts on food safety by minimizing preharvest contamination entering the food chain. laboratory-controlled studies determined the effects of soil ph, moisture content, and soil organic matter (som) on the survivability of this pathogen through the creation of single-parameter gradients. longitudinal field-based ... | 2015 | 25934621 |