| detection of escherichia coli o157:h7 from musca domestica (diptera: muscidae) at a cattle farm in japan. | enterohemorrhagic escherichia coli (ehec) o157:h7 was isolated for the first time from musca domestica l. a total of 310 fly samples was collected from 4 different farms in obihiro-city, hokkaido, in the summer and autumn of 1997;5 samples carried e. coli serotype o157:h7. using elisa and vero cell cytotoxicity assay, 3 isolates from 1 cattle farm produced both active shiga-toxin type 1 (stx1) and 2 (stx2). these isolates also carried hemolysin and eaea genes and harbored the 90-kb virulence pla ... | 1999 | 10071501 |
| molecular epidemiological study on tetracycline resistance r plasmids in enterohaemorrhagic escherichia coli o157:h7. | restriction patterns obtained with ecori and southern hybridization were used for the differentiation of tetracycline-resistant (tet(r)) r plasmids in enterobaemorrhagic escherichia coli (ehec) o157:h7 isolates from a mass outbreak at a kindergarten in obihiro-city, hokkaido, japan, 1996. two kinds of tet(r) r plasmids of 50 and 95 kb were detected. the 50-kb plasmids were identical to each other, while the 93-kb plasmids were of three types that were very similar to each other. the tet genes of ... | 1999 | 10487638 |
| clonal structure of shiga toxin (stx)-producing and beta-d-glucuronidase-positive escherichia coli o157:h7 strains isolated from outbreaks and sporadic cases in hokkaido, japan. | a total of 22 clonal phenotypic variants of shiga toxin (stx)-producing escherichia coli (stec) o157:h7 was isolated from six different locations in hokkaido, japan. these isolates were negative for sorbitol fermentation but positive for beta-d-glucuronidase (gud+). they carried eaea, ehec-hlya, pas and etpd genes like typical e. coli o157:h7 and, in addition, st1 and stx2 genes. however, they were shown to lack katp and espp genes that are present in typical stec o157:h7. all these atypical gud ... | 2002 | 11990493 |