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screening procedure from cattle feces and the prevalence of escherichia coli o157:h7 in taiwan dairy cattle.a procedure has been established for screening escherichia coli o157:h7 from bovine feces. it consists of four steps: enrichment, selective culture, phenotyping and genotyping. modified trypticase soy broth (mtsb) containing 20 microg/ml of novobiocin was used for the enrichment step. the selective culture step was done using sorbitol macconkey agar containing 0.05 microg/ml of cefixime and 2.5 microg/ml of potassium tellurite. the phenotyping steps include: species confirmation as e. coli, sero ...200111321124
one-year (2003) nationwide pork carcass microbiological baseline data survey in taiwan.from january through december 2003, swab samples from 1,650 pork carcasses were collected from 39 slaughter plants in taiwan. these samples were analyzed for the prevalence of indicator microorganisms and specific pathogens. viable aerobic bacteria, total coliforms, and escherichia coli were recovered from 100, 95.3, and 87.5% of these carcasses, respectively. of those carcasses that harbored bacteria, the mean aerobic plate, total coliform, and escherichia coli counts were 4.0, 0.6, and 0.1 log ...200515771166
isolation and identification of escherichia coli o157:h7 in a taiwanese patient with bloody diarrhea and acute renal failure.escherichia coli o157:h7 is a frequent foodborne pathogen in many developed countries. outbreaks of this infection have been reported in countries all over the world. the first clinical case of e. coli o157:h7 infection from taiwan was reported in a 6- year-old boy who had returned from usa in august 2001. in this paper, we describe the results of the isolation and identification of this strain, and molecular typing for comparison with previously reported strains. biochemical and molecular biolo ...200515818438
molecular subtyping for escherichia coli o157: h7 isolated in taiwan.enterohaemorrhagic escherichia coli o157: h7 is an important pathogen these days. outbreaks of its infection have been reported all over the world, in australia, canada, japan, the united states, south africa, and various countries in europe. in the summer of 2001, the first clinical infection by e. coli o157: h7 was identified in taiwan. in this study, the standard procedures for molecular subtyping were applied to several strains collected in taiwan as well as from elsewhere. the two molecular ...200516034200
characterization of bacterial susceptibility isolates in sixteen dairy farms in taiwan.bacteria were isolated from dairy cows, dairy farm environments, and dairy workers in 2 geographically different areas of eastern and northern taiwan. isolates were evaluated for antimicrobial susceptibility and the phylogenetics of isolated escherichia coli o157:h7 were characterized. a total of 1,346 bacteria were identified, including 226 e. coli, 30 pseudomonas spp. (7 pseudomonas aeruginosa), 259 other gram-negative bacteria, 271 enterococcus spp., 314 staphylococcus spp., 195 streptococcus ...200617106089
physiological response and protein expression under acid stress of escherichia coli o157:h7 twc01 isolated from taiwan.escherichia coli o157:h7 has an unusually high resistance to acidic environments. some research has revealed that acid-adapted cells, by exposure to moderately acidic conditions, are more resistant to a subsequent strong acidic challenge or other stress. this study was conducted to understand the protein expression regulation of acid tolerance response (atr) of a local isolated e. coli o157:h7 twc01 (twc01) induced by an acidic environment. twc01 cells were acid adapted by using hydrochloric aci ...200717630766
pathogenic microbiological baseline survey of pork carcasses in taiwan.from 2004 to 2010, pork carcass swabs from state-inspected slaughter plants in taiwan were intermittently analyzed to determine the prevalence of selected pathogenic microorganisms associated with foodborne illness. the prevalences of staphylococcus aureus each year from 2006 to 2010 were 6.6, 10.8, 5.1, 6.4, and 7.4%, respectively, while those of listeria monocytogenes were 1.2% in 2004, 1.3% in 2005, and 3.5% in 2008. the prevalences of clostridium perfringens were 0.9% in 2004, 3.2% in 2005, ...201323726203
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