molecular study of echinococcus in west-central china.west-central china is an important endemic focus of both alveolar and cystic echinococcosis where several species of intermediate host are commonly infected with echinococcus granulosus and e. multilocularis . isolates of e. granulosus were collected from humans and other animals from different geographical areas of qinghai, ningxia, gansu and sichuan, and genotyped using the mitochondrial dna marker atp synthase subunit 6 gene (atp6). the sheep strain (g1 genotype) of e. granulosus was shown to ...200516174420
assessment of echinococcus granulosus polymorphism in qinghai province, people's republic of china.cystic echinococcosis (ce) is highly endemic in the chinese province of qinghai, located on the tibetan plateau. the echinococcus granulosus sheep strain has already been reported in this focus. to improve our understanding of the role the parasite plays in the high prevalence observed in humans, we assessed the genetic polymorphism of 55 e. granulosus samples (37 from humans) using three discriminative mitochondrial markers: coxi, nadi and atp6. we obtained a total of 13 distinct genotypes whic ...200818309521
species identification of human echinococcosis using histopathology and genotyping in northwestern china.human cystic echinococcosis, caused by infection with the larval stage of echinococcus granulosus, and alveolar echinococcosis, caused by the larval form of e. multilocularis, are known to be important public health problems in western china. echinococcus shiquicus is a new species of echinococcus recently described in wildlife hosts from the eastern tibetan plateau and its infectivity and/or pathogenicity in humans remain unknown. in the current study, parasite tissues from various organs were ...200818396303
cystic and alveolar echinococcosis: an epidemiological survey in a tibetan population in southeast qinghai, epidemiological investigation on echinococcosis was made in jiuzhi county of qinghai province, western china. ultrasonography and an indirect hemagglutination test revealed a morbidity of 8.0% (124/1,549) and a seroprevalence of 25.8% (287/1,113), respectively, in the tibetan population. the morbidity in herdsmen (16.6%) and buddhist priests (15%) was significantly higher than that in other occupation groups (3.2%), and it was higher in females (9.8%) than in males (6.2%). the ultrasound imag ...200818503183
development of three pcr assays for the differentiation between echinococcus shiquicus, e. granulosus (g1 genotype), and e. multilocularis dna in the co-endemic region of qinghai-tibet plateau, investigate echinococcosis in co-endemic regions, three polymerase chain reaction (pcr) assays based on the amplification of a fragment within the nadh dehydrogenase subunit 1 (nd1) mitochondrial gene were optimized for the detection of echinococcus shiquicus, echinococcus granulosus g1, and echinococcus multilocularis dna derived from parasite tissue or canid fecal samples. specificity using parasite tissue-derived dna was found to be 100% except for e. shiquicus primers that faintly detecte ...201323438764
[an epidemiological survey on echinococcosis in zhiduo county of qinghai province].the survey was carried out in july, 2006 in zhiduo county. the iha and elisa positive rate in human population was 4.5% (42/933) and 8.2% (76/931) respectively. ultrasonography revealed a morbidity of 3.4% (33/979) with 3.2% echinococcus granulosus and 0.2% of e. multilocularis respectively. animal dissection showed an infection rate of 15.1% (14/93) in pikas with one infected by e. shiquicus proved by molecular biology. coproantigen rate by elisa was 62% (12/193) in dogs and 35.7% (5/14) in wol ...200718038785
dual infection of animal hosts with different echinococcus species in the eastern qinghai-tibet plateau region of china.the eastern qinghai-tibet plateau of china is a highly endemic region of echinococcosis where echinococcus granulosus sensu stricto (sheep strain), echinococcus multilocularis, and echinococcus shiquicus are distributed sympatrically. we developed a polymerase chain reaction-based restriction fragment length polymorphism (pcr-rflp) method for the identification of the three species in this region. the pcr-rflp showed the dual infection of animals with different echinococcus spp. the first case w ...200616896135
echinococcus shiquicus, a new species from the qinghai-tibet plateau region of china: discovery and epidemiological shiqu county of the qinghai-tibet plateau, many wild and domestic mammals are involved in the transmission cycles of echinococcus spp. echinococcus multilocularis and echinococcus granulosus genotype g1 (sheep strain) are sympatrically distributed in the plateau. in 1995, we identified a unique strobilate stage of echinococcus from the tibetan fox, vulpes ferrilata, but considered it to be a variant of e. multilocularis. subsequent molecular genetic studies revealed that a hydatid cyst from t ...200616337180
echinococcosis on the tibetan plateau: prevalence and risk factors for cystic and alveolar echinococcosis in tibetan populations in qinghai province, china.infections by larval stages of tapeworms of the genus echinococcus (echinococcosis or hydatid disease) are zoonotic infections of major public health importance throughout much of the world. humans become infected through accidental ingestion of eggs passed in faeces of canid definitive hosts. tibetan populations of china have some of the highest documented levels of infections by both echinococcus granulosus and e. multilocularis, the causes of cystic and alveolar echinococcosis, respectively. ...200315027608
short report: identification of echinococcus species from a yak in the qinghai-tibet plateau region of china.the species identification of an echinococcal lesion in the liver of a yak in the qinghai-tibet plateau region of china, where both echinococcus granulosus and e. multilocularis are present, was difficult to determine because of the atypical appearance of the lesion. polymerase chain reaction-based mitochondrial genotyping allowed us to discriminate the echinococcus species. nucleotide sequences of the cytochrome c oxidase subunit 1 (cox1) and cytochrome b (cytb) genes amplified from the echinoc ...200314640506
[rflp analysis of dna from echinococcus granulosus collected from four provinces/autonomous region in china].a fragment of the ribosomal rna gene of schistosoma mansoni psm889 and two dna fragments specific to echinococcus granulosus phd5 and peg18 have been used as dna probes to assess the extent of genetic variability within e. granulosus. the dna analysis, including restriction endonuclease digestion and southern blot hybridization with the probes, did not demonstrate any genetic variation among e. granulosus collected from sheep in xinjiang, qinghai, gansu and ninxia. similarly, there was no geneti ...19937909504
epidemiological studies on cystic echinococcosis in china--a the four decades from 1951 to 1990, the six provinces or autonomous regions (xinjiang, gansu, qinghai, ningxia, xizang and nei monggol) reported a total of 26,065 surgical cases of hydatid disease, most of which were reported in the recent decade. about one third of the patients was children and adolescents under 15 years old. so far, cystic hydatid infections of local origin have been confirmed in 22 provinces, autonomous regions and municipalities in the whole nation. findings of x-ray exam ...19957546341
epidemiological investigation and risk factors of echinococcus granulosus in yaks (bos grunniens), tibetan pigs and tibetans on qinghai tibetan plateau.echinococcus granulosus (e. granulosus) is a diverse zoonotic parasite and causes cystic echinococcosis (ce) disease in humans and livestock. however, scare information is available about the epidemic situation of e. granulosus infection in yaks, tibetan pigs and native tibetans on the qinghai tibetan plateau. therefore, a study was carried out to find prevalence and risk factors of e. granulosus in yaks, tibetan pigs and tibetans. serum samples from yaks (1371), tibetan pigs (454) and tibetans ...201728624512
molecular identification of echinococcus species from eastern and southern qinghai, china, based on the mitochondrial cox1 gene.the qinghai-tibetan plateau (qtp, in western china), which is the largest and highest plateau on earth, is a highly epidemic region for echinococcus spp. we collected 70 echinococcus samples from humans, dogs, sheep, yaks, plateau pikas, and voles in eastern and southern qinghai and genotyped them using the mitochondrial dna marker cytochrome oxidase subunit i gene and maximum parsimony and bayesian reconstruction methods. based on the 792-bp sequence matrix, we recorded 124 variable sites, of w ...201222258080
genotypes of echinococcus granulosus in animals from yushu, northeastern china.echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance worldwide. the aim of this study was to determine the genotype distribution of e. granulosus in animals in yushu, qinghai province, northeastern china. the mitochondrial nicotinamide adenine dinucleotide+hydrogen (nadh) dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) genes from 30 echinococcosis isolates were analyzed by sequence alignment, generating two unique ...201323289393
epidemiological study and control trial of taeniid cestode infection in farm dogs in qinghai province, epidemiological study and control trial were conducted to assess taeniid infection in farm dogs in qinghai province, china. to improve egg detection by fecal examination, a deworming step with praziquantel was incorporated into the sampling methodology. as a result, a marked increase in the number of egg-positive samples was observed in samples collected at 24 hr after deworming. then, the fecal examination and barcoding of egg dna were performed to assess the prevalence of taeniid species in ...201424257329
discrimination between e. granulosus sensu stricto, e. multilocularis and e. shiquicus using a multiplex pcr assay.infections of echinococcus granulosus sensu stricto (s.s), e. multilocularis and e. shiquicus are commonly found co-endemic on the qinghai-tibet plateau, china, and an efficient tool is needed to facilitate the detection of infected hosts and for species identification.201526393793
surveillance of echinococcus isolates from qinghai, china.echinococcosis is highly endemic over large parts of the qinghai-tibet plateau (qtp), china. based on a large number of samples, we present data on the current presence, host distribution, and genetic diversity of echinococcus in the qinghai province, located in the northeastern corner of the qtp and constituting >25% of the area of the plateau. we used 521 samples (including 451 newly collected samples and 70 samples from our previous study) from humans, yaks, sheep, goats, dogs, fox, plateau p ...201525480467
reverse vaccinology approach identify an echinococcus granulosus tegumental membrane protein enolase as vaccine candidate.applying reverse vaccinology strategy, we employed a sequence encoding an enolase from taenia asiatica to search its homolog in the expression sequence tag (est) database of echinococcus granulosus and found two est sequences (access number: cn653186 and cn649593) of a clone eg_psgrs_13b09 from e. granulosus protoscolex full-length cdna library, which are responding for the 5' and 3' partial cds of e. granulosus enolase, respectively. primers are designed according to the 5' end and 3'end of the ...201020127115
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