identification and biochemical characterization of leishmania strains isolated in peru, mexico, and spain.eight leishmania promastigotes were isolated from different geographical areas: three (lp1, lp2, and lp3) from the provincial department la libertad and the fourth (lp4) from the department of cajamarca (northern peru); another three (lm1, lm2, and lm3) in the province of campeche (mexico); and the last (ls1) from a clinical case of a dog in madrid (spain). the isolates were characterized by carbohydrate cell-surface residues using agglutinations with four purified lectins, by isoenzyme analysis ...200616289175
molecular probes and the polymerase chain reaction for detection and typing of leishmania species in mexico.leishmaniasis in mexico is a public health problem because all the clinical forms have been recorded in most mexican states. we studied patients showing clinical symptoms of any form of leishmaniasis, from several endemic areas. bone marrow samples, aspirates or skin biopsies were taken and deoxyribonucleic acid (dna) was extracted and amplified by the polymerase chain reaction (pcr) with universal primers ajs1 and deb8, specific for the leishmania subgenus leishmania. the pcr products were then ...200212055821
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