aetiology of visceral leishmaniasis in mexico.two children with visceral leishmaniasis (vl), were studied by dna analysis. dna from liver biopsy samples from both patients, was amplified by pcr with broad primers specific for the leishmania subgenus. dna from the patient from chiapas was also amplified with primers specific for the leismania donovani complex and hybridised with a probe specific for l. donovani complex. the second patient, who is the first reported case of visceral leishmaniasis in the mexican state of tabasco, where localis ...200010708655
molecular probes and the polymerase chain reaction for detection and typing of leishmania species in mexico.leishmaniasis in mexico is a public health problem because all the clinical forms have been recorded in most mexican states. we studied patients showing clinical symptoms of any form of leishmaniasis, from several endemic areas. bone marrow samples, aspirates or skin biopsies were taken and deoxyribonucleic acid (dna) was extracted and amplified by the polymerase chain reaction (pcr) with universal primers ajs1 and deb8, specific for the leishmania subgenus leishmania. the pcr products were then ...200212055821
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