risk factors for leishmania chagasi infection in an urban area of minas gerais order to understand the determinants of human infection by leishmania chagasi in an urban area, a cross-sectional population based study was conducted using molecular and serologic methods to identify infection. participants were interviewed using a pre-coded questionnaire. two criteria were tested to identify risk factors: model 1--including all participants positive in hybridization by leishmania donovani complex probe; model 2--including all participants positive for hybridization and at l ...200616410918
diagnosing human asymptomatic visceral leishmaniasis in an urban area of the state of minas gerais, using serological and molecular biology techniques.a population-based cross-sectional study was set up in sabará country, southeastern brazil, to identify asymptomatic human visceral leishmaniasis in an urban area of low disease prevalence. blood was collected on filter paper (n=1,604 inhabitants) and examined by indirect immunofluorescent test, enzyme-linked immunosorbent assay and immunochromatographic strip test. the prevalence rates of infection ranged from 2.4 to 5.6% depending on the test used. one year later, venous blood was collected in ...200617160317
histopathological and parasitological investigations of ear healthy skin of dogs naturally and experimentally infected with leishmania (leishmania) chagasi.although 90% of clinical cases of american visceral leishmaniasis (avl) occur in the northeastern region of brazil, the incidence of cases in recent years has increased in southeastern states such as minas gerais (mg), where the disease has been reported in several cities, including belo horizonte, the state capital. some studies have shown a strong correlation between the incidence of avl and canine visceral leishmaniasis (cvl) in belo horizonte. a study of 108 dogs with parasite leishmania cha ...201020503176
parasitological diagnosis of canine visceral leishmaniasis: is intact skin a good target?the objective of this study was to evaluate intact skin of seroreactive dogs as a possible target for the parasitological confirmation of canine visceral leishmaniasis (cvl). for this purpose, 394 dogs identified in serological surveys carried out in the metropolitan region of belo horizonte were studied. blood was collected from all animals for serology and a tissue sample was obtained from two sites for parasitological diagnosis. skin obtained from the ear and scapular region was simultaneousl ...200919364614
leishmaniasis in brazil. xix: visceral leishmaniasis in the amazon region, and the presence of lutzomyia longipalpis on the island of marajó, pará state.sporadic cases of visceral leishmaniasis in amazonian brazil appear limited to pará state, in the lower amazon valley and principally near the atlantic coast. the fox cerdocyon thous (l.) has been incriminated as a natural host of the causative parasite, leishmania donovani chagasi, but past doubts have existed over the identification of the most likely vector as lutzomyia (lutzomyia) longipalpis (lutz & neiva, 1912). investigations on two of five recent cases of visceral leishmaniasis of man in ...19836623589
identification and distribution of new world leishmania species characterized by serodeme analysis using monoclonal antibodies.five hundred thirty stocks of leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of american cutaneous (acl) and visceral leishmaniases (avl) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. this study confirms and extends our preliminary results on the high specificity of some of these ...19873826486
immunogenicity assay of the leishmune vaccine against canine visceral leishmaniasis in brazil.leishmune is the industrialized version of the fml-saponin vaccine which has been shown to develop 92-95% protection in vaccinated dogs and 76-80% vaccine efficacy against field canine visceral leishmaniasis (cvl) in brazil. leishmune has been proven to be safe and tolerable and a transmission-blocking vaccine which renders vaccinated dogs non-infectious to sand fly vectors. in the present investigation, 550 healthy seronegative dogs of endemic and epidemic areas of brazil were monitored for lei ...200818675868
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