a survey for american cutaneous and visceral leishmaniasis among 1,342 dogs from areas in rio de janeiro (brazil) where the human diseases occur.there are areas in the periphery of rio de janeiro city where human cases of visceral and/or cutaneous leishmaniasis occur. the parasites have been identified as leishmania donovani and leishmania braziliensis braziliensis respectively. a survey for leishmaniasis was done among 1,342 dogs from those areas using an indirect immunofluorescent test. from the dogs, 616 came from areas where only human cases of visceral leishmaniasis occurred, 373 from an area where all human cases were of cutaneous ...19853910994
[comparative validation study between the elisa and rifi techniques for diagnosing leishmania sp in stray dogs caught in the municipality of campos de goytacazes, state of rio de janeiro].a survey was carried out aiming to verify the elisa test effectiveness for detecting antibodies against leishmania sp in dogs, comparing with rifi human pattern and for investigating sorological zoonosis situation in the microregion. an accordance about 97.6% considered strong was reported.200717876477
canine visceral leishmaniasis in rio de janeiro, brazil. clinical, parasitological, therapeutical and epidemiological findings (1977-1983).forty dogs from the periphery of the city of rio de janeiro were studied. all dogs where diagnosed as positive for leishmaniasis either parasitologically and/or serologically. among them, 19 came from areas where only visceral leishmaniasis (vl) occurs (realengo, bangu, senador camará). clinical signs of the disease were seen in 36.8% of the cases, including emaciation - 100%, lymphadenopathy and depilation - 85.7%. the other 21 dogs came from an area (campo grande) where both diseases (vl, and ...19853837171
active cutaneous leishmaniasis in brazil, induced by leishmania donovani chagasi.l.d. chagasi was isolated from active cutaneous leishmaniasis in both human and canine infections in an endemic area in rio de janeiro, brazil. both isolates were identified by molecular and immunological characterization of the parasite using three different methods: electrophoretic mobility of isoenzymes; restriction endonuclease fragment analysis of kdna and serodeme analysis using monoclonal antibodies. this seems to be the first well documented case in the new world of a "viscerotropic" lei ...19863574128
prevalence of anti-leishmania donovani antibody among brazilian blood donors and multiply transfused hemodialysis patients.the prevalence of anti-leishmania donovani antibodies was investigated in 1,500 brazilian blood donors and multiply transfused hemodialysis patients. sera were tested using the fucose-mannose ligand (fml) elisa, which was shown to have 100% sensitivity and 96% specificity for kala-azar. among 1,194 volunteer blood donors, seroreactivity was 9%, increasing to 25% in a periurban kala-azar focus. however, higher positivity (37%) was found in multiply transfused hemodialysis patients from natal, whe ...19979288810
identification and distribution of new world leishmania species characterized by serodeme analysis using monoclonal antibodies.five hundred thirty stocks of leishmania isolated from human and domestic and wild reservoir hosts, representing a wide geographic distribution of endemic foci of american cutaneous (acl) and visceral leishmaniases (avl) were characterized and identified at species and/or subspecies levels based on their reactivity to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. this study confirms and extends our preliminary results on the high specificity of some of these ...19873826486
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