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restriction fragment length polymorphism (rflp) analysis of genomic dna of 5 strains of trichinella spiralis in china.five restriction endonucleases were used to digest genomic dna from 5 isolates of trichinella spiralis obtained from changchun, tianjin, xian, henan and yunnan. all the isolates were secured from pigs except the changchun strain which came from dog. the dna fragments digested by endonuclease were separated by agarose gel electrophoresis. the changchun isolate had a ecori band at 1.12kb and a drai band at 1.97kb which were unique to this isolate. a cloned specific repetitive dna sequence (1.12kb) ...19958580480
analysis of chinese isolates of trichinella spiralis by molecular biotechnology.to investigate the relationships among trichinella spiralis isolates obtained from different hosts and geographical regions in china.19969275332
pcr-sscp of rdna for the identification of trichinella isolates from mainland china.a polymerase chain reaction (pcr)-based single strand conformation polymorphism (sscp) analysis of the expansion segment 5 (domain iv) of the large subunit of ribosomal dna was employed to characterize seven isolates of trichinella from china (a-g), including six of pig origin from regions in dengxian (a), tianjin (b), harbin (d), baoshan (e), xinye (f) and xian (g), and one of canine origin from changchun (c). isolates a, d, e, f and g were classified as trichinella spiralis based on sscp patte ...19989584075
outbreaks of human trichinellosis caused by consumption of dog meat in china.dog meat has become an important source of trichinella infection for humans in china. the first documented outbreak of human trichinellosis resulting from the consumption of dog meat occurred in china in 1974. until 1999, the outbreaks with this source of infection occurred mostly in northeast of china (81 cases in five outbreaks in jilin and two in liaoning), beijing (six cases) and henan provinces (two cases). the epidemiological surveys were performed in nine provinces or autonomous regions o ...200111484389
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