| arbitrarily primed polymerase chain reaction provides rapid differentiation of proteus mirabilis isolates from a pediatric hospital. | during a systematic survey, maternal carriage of proteus mirabilis was found over a 25-day period in 18 pregnant women admitted to the delivery ward of our hospital maternity. five neonates born to these mothers were found to be colonized with p. mirabilis. we report here on the use of dna fingerprinting by the arbitrarily primed polymerase chain reaction technique (ap-pcr) for the epidemiological investigation of this sudden outbreak. this approach was compared with the analysis of restriction ... | 1993 | 8099079 |
| diversity of ctx-m beta-lactamases and their promoter regions from enterobacteriaceae isolated in three parisian hospitals. | nine clinical isolates of enterobacteriaceae (six escherichia coli and three proteus mirabilis) isolated in three parisian hospitals between 1989 and 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime and aztreonam but not to ceftazidime. ctx-m-1, ctx-m-2, ctx-m-9, ctx-m-14 and two novel plasmid-mediated ctx-m beta-lactamases (ctx-m-20, and ctx-m-21) were identified by polymerase chain reaction and isoelectric focusing (pi>8) and ... | 2002 | 12007800 |
| spread of novel expanded-spectrum beta-lactamases in enterobacteriaceae in a university hospital in the paris area, france. | in 2002, 28 non-duplicate enterobacterial isolates producing extended-spectrum beta-lactamases (esbls) were collected from infected patients at the bicêtre hospital in paris, france. escherichia coli was the predominant esbl-positive enterobacterial species, comprising ten (36%) of the isolates. ctx-m enzymes (ctx-m-3, ctx-m-10, ctx-m-14 and ctx-m-15) were produced by 11 (39%) of the isolates (six e. coli, two enterobacter cloacae, one enterobacter aerogenes, one proteus mirabilis and one citrob ... | 2005 | 15966981 |
| occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005. | bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ... | 2006 | 16961639 |
| characterization of cmy-type beta-lactamases in clinical strains of proteus mirabilis and klebsiella pneumoniae isolated in four hospitals in the paris area. | we isolated five clinical strains (three proteus mirabilis and two klebsiella pneumoniae) with beta-lactam resistance phenotypes consistent with production of an ampc-type beta-lactamase. the predicted amino acid sequences of the enzymes were typical of class c beta-lactamases. the enzymes were identified as cmy-2, cmy-4 and a new cmy-variant beta-lactamase, cmy-12. the ampc beta-lactamases from the two k. pneumoniae isolates were found to be encoded on self-transferable plasmids. the genes enco ... | 2002 | 12407124 |