| [legionella infections in the paris area. predominance of serogroup 1 legionella pneumophila infections]. | | 1985 | 3158958 |
| monoclonal antibody reactivity as a virulence marker for legionella pneumophila serogroup 1 strains. | using a panel of nine monoclonal antibodies, we subgrouped 85 environmental and 129 clinical legionella pneumophila serogroup 1 isolates from paris, france. patients were unlikely to be epidemiologically linked either with each other or with the 44 sampled environmental sites (14 air conditioning systems and 30 buildings) that were selected at random in the paris area. according to their monoclonal antibody patterns, isolates belonged to 14 subgroups. monoclonal antibody 2 recognized 121 (93.8%) ... | 1988 | 3343524 |
| single clonal origin of a high proportion of legionella pneumophila serogroup 1 isolates from patients and the environment in the area of paris, france, over a 10-year period. | arbitrarily primed pcr with three primers and pulsed-field gel electrophoresis were used to characterize a set of 75 clinical legionella pneumophila serogroup 1 isolates, with no apparent epidemiological link, obtained from 24 hospitals in paris, france, from 1987 to 1997. unexpectedly, 25 clinical isolates from 15 hospitals had an identical profile (termed type a) by both methods. the same profile was subsequently found in 16 of 64 randomly selected environmental l. pneumophila serogroup 1 isol ... | 1999 | 10405416 |
| legionella pneumophila serogroup 1 strain paris: endemic distribution throughout france. | an analysis of 691 french clinical legionella isolates showed that the endemic l. pneumophila serogroup 1 strain paris was responsible for 12.2% of all cases of legionellosis and had a specific pulsed-field gel electrophoresis pattern. we also demonstrated the presence of this endemic clone throughout europe. | 2003 | 12843082 |
| molecular typing of nosocomial isolates of legionella pneumophila serogroup 3. | in paris, france, an outbreak of pneumonia due to legionella pneumophila serogroup 3 was observed in necker (four cases) and pitié (six cases) hospitals. neither the 10 clinical isolates nor 5 tap water isolates from necker hospital harbored plasmids. clinical and environmental serogroup 3 isolates and serogroup 3 reference strain bloomington 2 were analyzed by chromosomal probe fingerprinting. rrna, 16s and 23s from escherichia coli and a randomly cloned 15-kilobase-pair nucleotide sequence fro ... | 1990 | 2179256 |
| validation of a microbead-based format for spoligotyping of legionella pneumophila. | a 42-plex clustered regularly interspaced short palindromic repeat (crispr)-based typing technique (spoligotyping) was recently developed at the french national reference center for legionella. it allows the subtyping of the legionella pneumophila sequence type 1/paris pulsotype. in this report, we present the transfer of the membrane-based spoligotyping technique to a microbead-based multiplexed format. this microbead-based high-throughput assay uses devices such as luminex 200 or the recently ... | 2014 | 24759720 |
| legionella pneumophila st1/paris-pulsotype subtyping by spoligotyping. | endemic strains of legionella pneumophila st1, in particular the st1/paris-pulsotype, are dispersed worldwide and represent about 10% of culture-proven clinical cases of ld in france. the high isolation rate of this strain, in both clinical and environmental samples, makes identification of the source of infection difficult during epidemiological investigations. the full-length genome sequence of this strain was recently determined and it revealed the presence of a crispr/cas complex. the aim of ... | 2011 | 22205819 |
| insertion sequences as highly resolutive genomic markers for sequence type 1 legionella pneumophila paris. | the causative agent of legionellosis, legionella pneumophila, colonizes all natural and human-made water networks, thus constituting the source of contaminated aerosols responsible for airborne human infections. efficient control of infections, especially during epidemics, necessitates the fastest and most resolutive identification possible of the bacterial source for subsequent disinfection of reservoirs. we thus compared recognized typing approaches for legionella with a method based on charac ... | 2010 | 20980561 |
| host-related risk factors and clinical features of community-acquired legionnaires disease due to the paris and lorraine endemic strains, 1998-2007, france. | in france, legionnaires disease is mainly caused by legionella pneumophila. here, we investigated possible host factors associated with susceptibility to community-acquired legionnaires disease caused by the endemic paris and lorraine strains. | 2009 | 19508168 |
| legionella pneumophila goes clonal--paris and lorraine strain-specific risk factors. | | 2009 | 19508166 |
| [legionnaires disease : 3 cases from the northern suburbs of paris (author's transl)]. | during the summer of 1980, 3 sporadic cases of legionnaires disease (ml) were recognized in the northern suburbs of paris. the clinical picture was characterized by an extensive pneumonia, high fever with repeated rigors (3 cases), a confusional state (2 cases), and transitory watery diarrhea (3 cases). blood cultures, evidence of bacterial antigens in blood or urine, and serology notably for chlamydia and mycoplasma pneumoniae were all negative. the diagnosis of ml (serotype i) was confirmed by ... | 1981 | 7302345 |
| nosocomial legionellosis, paris, france. evidence for transmission by potable water. | during a five-week period in 1981, six cases of legionellosis due to legionella pneumophila serogroup 1 were recognized in a hospital in paris, france. four cases were clearly nosocomial in origin. there was a direct association between development of disease and exposure to potable hot water (p = 0.003). the entire hot water system was contaminated with l. pneumophila serogroup 1; monoclonal antibody testing demonstrated that the case isolate and the potable water isolates belonged to the same ... | 1985 | 3985035 |
| the intracellular multiplication of legionella pneumophila in protozoa from hospital plumbing systems. | between october 1987 and march 1989, we tested 144 water samples obtained from the plumbing and cooling tower systems of 5 paris hospitals for the presence of legionellae and amoebae. of the samples tested for legionella, 67 out of 144 (46.5%) were positive, and 82 out of 116 tested for amoebae (70.7%) were positive. the ability of protozoa to support the multiplication of legionella was shown by incubating samples at 35.5 degrees c for 7-15 days. prior to determining the presence of legionellae ... | 1991 | 1961979 |