molecular epidemiology of classical swine fever in gain an insight into the molecular epidemiology of classical swine fever (csf) in italy, virus isolates originating from outbreaks that occurred between 1985 and 2000 in wild boar or in domestic pigs in mainland italy and in sardinia were analysed by genetic typing. for this, a fragment (190 nucleotides) of the e2 glycoprotein gene was sequenced and phylogenetic analyses were performed, including older italian isolates and isolates from recent outbreaks in europe for comparison. the results s ...200111574170
classical swine fever in sardinia: epidemiology of recent outbreaks.a variable region of the gene encoding the major glycoprotein (e2) of classical swine fever virus (csfv) was sequenced from 12 sardinian isolates which had been obtained from three geographically distinct regions of the island. phylogenetic analysis of these viruses and others characterized in previous studies [1, 2] indicated that (a) the sardinian viruses were all members of the common european subgroup 2.3 and were clearly distinct from live vaccines recently used in this area; (b) they could ...199910459661
effect of husbandry methods on seropositivity to african swine fever virus in sardinian swine herds.multiple logistic regression was used on serological data collected in the context of the sardinian african swine fever (asf) eradication program from pig farms in the province of nuoro, sardinia. the monthly percentage of asfv-positive herds decreased significantly from october 1994 through march 1996 (p < 0.001). the farm-level risk of seropositivity to african swine fever virus (asfv) was higher in free-range farms than in partial-confinement farms (odds ratios (or) varied between 4.9 in octo ...19979443330
epidemiology of classical swine fever in sardinia: a serological survey of wild boar and comparison with african swine fever.a serological survey was carried out to establish the distribution of classical swine fever among wild boar in sardinia, where that disease and african swine fever have been endemic in free-ranging domestic pigs and wild boar living in the mountainous areas of the province of nuoro for several years. blood samples were collected from 4752 wild boar shot during the period december 1988 to january 1992. an overall prevalence of 11 per cent was observed and the almost constant rate of about 9.8 per ...19948171792
genetic characterisation of african swine fever viruses from recent and historical outbreaks in sardinia (1978-2009).three discrete regions of the african swine fever virus (asfv) were analysed in the genomes of a wide range of isolates collected from wild and domestic pigs in sardinia, over a 31-year period (1978-2009). the analysis was conducted by genotyping based on sequence data from three single copy asf genes. the e183l gene encoding the structural protein p54 and part of the gene encoding the p72 protein were used to delineate genotypes, before intra-genotypic resolution of viral relationships by analy ...201121373994
improved strategy for molecular characterization of african swine fever viruses from sardinia, based on analysis of p30, cd2v and i73r/i329l variable regions.african swine fever virus (asfv) is the aetiological agent of a highly lethal haemorrhagic disease affecting pigs that inflicts significant economic damage on the swine industry. asf is present in many african countries, in several eastern and central european countries and in sardinia (italy). sequence analyses of variable genomic regions have been extensively used for molecular epidemiological studies of asfv isolates. previous sequencing data of genes that codify for viral protein p54, p72 an ...201627174805
understanding african swine fever infection dynamics in sardinia using a spatially explicit transmission model in domestic pig farms.african swine fever virus (asfv) has been endemic in sardinia since 1978, resulting in severe losses for local pig producers and creating important problems for the island's veterinary authorities. this study used a spatially explicit stochastic transmission model followed by two regression models to investigate the dynamics of asfv spread amongst domestic pig farms, to identify geographic areas at highest risk and determine the role of different susceptible pig populations (registered domestic ...201728296281
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