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porcine circovirus type 2 (pcv2) infection decreases the efficacy of an attenuated classical swine fever virus (csfv) vaccine.abstract: the lapinized philippines coronel (lpc) vaccine, an attenuated strain of classical swine fever virus (csfv), is an important tool for the prevention and control of csfv infection and is widely and routinely used in most csf endemic areas, including taiwan. the aim of this study was to investigate whether pcv2 infection affects the efficacy of the lpc vaccine. eighteen 6-week-old, cesarean-derived and colostrum-deprived (cdcd), crossbred pigs were randomly assigned to four groups. a tot ...201122129109
complete sequence of a subgroup 3.4 strain of classical swine fever virus from taiwan.classical swine fever viruses from taiwan have been classified into two subgroups (3.4 and 2.1). outbreaks caused by 3.4 viruses were reported in taiwan prior to 1996 and which mainly distributed in the geographic range from southern japan to taiwan. we have determined the complete sequence of a reference strain, 94.4/il/94/twn. the genome contains 12,296 nucleotides, encoding 3,898 amino acids flanked by a 372-nt region at the 5' untranslated region (utr) and a 227-nt region at the 3'-utr. simi ...200717721814
molecular cloning and nucleotide sequence determination of three envelope genes of classical swine fever virus taiwan isolate p97.a strain of classical swine fever virus (csfv) has been isolated in taiwan. the cdna coding for three envelope glycoproteins e1, e2 and e3 were molecularly cloned from purified viral particles using the reverse transcription-polymerase chain reaction (rt-pcr) method and sequence-specific primers. the resulting pcr products (1113 bp for e1. 699 bp for e2 and 567 bp for e3) were cloned into the smai site of puc19 and then subjected to dna sequence analysis. data showed that nucleotide sequence of ...19968738176
phylogenetic analysis of classical swine fever virus isolated from taiwan.by analyzing the e2 sequences of classical swine fever virus from field outbreaks in taiwan during 1993-2001, three virus populations with distinct genotypes were determined including one historical (subgroup 3.4) and two exotic (subgroup 2.1) strains. the first subgroup 2.1 virus was isolated in 1994 and further sporadic outbreaks occurred after 1996. phylogenetic analysis using the e2 region has segregated the taiwanese strains of 2.1 virus into two different genotypes (termed 2.1a and 2.1b). ...200515778024
phylogenetic analysis of classical swine fever virus in taiwan.two envelope glycoprotein (erns and e2) regions of the classical swine fever virus (csfv) were amplified by rt-pcr and sequenced directly from 158 specimens collected between 1989 and 2003 in taiwan. phylogenetic analysis of the two regions revealed a similar tree topology and the erns region provided better discrimination than the e2 region. one hundred and fifteen isolates out of the 158 isolates were clustered within subgroup 2.1 (further classified as 2.1a and 2.1b) and 2.2, which were consi ...200515703847
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