| detection of geographic isolates of anaplasma marginale, using polyclonal bovine antisera and microfluorometry. | geographically separate united states isolates of anaplasma marginale were differentiated, using polyclonal bovine antiserum and microfluorometry. both isolate-common and restricted antigen-specific antibodies were apparent in sera of splenectomized calves after resolution of infection, as judged by the capability of the antisera to recognize heterologous isolate antigens to a lesser extent than homologous antigens. furthermore, absorption of the antisera with homologous antigens removed homolog ... | 1985 | 3907437 |
| molecular and immunological characterization of three strains of anaplasma marginale grown in cultured tick cells. | anaplasma marginale is an economically important tick-borne pathogen of cattle that causes bovine anaplasmosis. a wide range of geographic strains of a. marginale have been isolated from cattle, several of which have been characterized using genomics and proteomics. while many of these strains have been propagated in tick lines, comparative analyses after propagation in tick cells have not been reported. the overall purpose of this research therefore was to compare the degree of conservation of ... | 2015 | 25943785 |
| anaplasma marginale msp1alpha genotypes evolved under positive selection pressure but are not markers for geographic isolates. | anaplasma marginale (order rickettsiales, family anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. many geographic isolates of a. marginale occur in the united states and have been identified by major surface protein 1a (msp1a), which varies in sequence and molecular weight due to different numbers of tandem 28- to 29-amino-acid repeats. the present study was undertaken to examine the genetic variations among isolates of a. marginale ... | 2003 | 12682152 |
| establishment and characterization of an oklahoma isolate of anaplasma marginale in cultured ixodes scapularis cells. | anaplasma marginale is a tick-borne hemoparasite of cattle worldwide. the virginia isolate of a. marginale was propagated previously in a cell line derived from embryos of the tick, ixodes scapularis. the cultured anaplasma (va-tc) was passaged continuously for over 4 years and retained its infectivity for cattle and antigenic stability. we report herein the continuous in vitro cultivation of a second isolate of a. marginale derived from a naturally infected cow in oklahoma (ok-tc). blood from t ... | 2000 | 10669100 |
| serologic and clinical responses of premunized, vaccinated, and previously infected cattle to challenge exposure by two different anaplasma marginale isolates. | two anaplasma marginale isolates, one originating in florida (fam) and the other from virginia (vam), were compared immunologically by cross-challenge exposure of 14 anaplasma carrier cattle, 8 previously infected cattle, and 6 splenectomized carrier calves. in addition, 28 cattle vaccinated with a commercially available adjuvant killed vaccine and 22 nonvaccinated cattle were challenge exposed with either fam or vam. a detectable clinical response was not produced by either fam or vam challenge ... | 1984 | 6084430 |
| flow cytometric evaluation of selected antimicrobial efficacy for clearance of anaplasma marginale in short-term erythrocyte cultures. | the tick-borne rickettsia, anaplasma marginale, causes the economically important cattle disease anaplasmosis. once infected, cattle remain lifelong carriers. herein, we used flow cytometry to test the efficacy of three antimicrobials; oxytetracycline, imidocarb and enrofloxacin against virginia (vgn) or oklahoma (ok) a. marginale isolates in short-term erythrocyte cultures. parasite viability was assessed using the vital dye hydroethidine (he), which is detectable when living organisms convert ... | 2006 | 16669861 |