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anaplasma marginale msp1alpha genotypes evolved under positive selection pressure but are not markers for geographic isolates.anaplasma marginale (order rickettsiales, family anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. many geographic isolates of a. marginale occur in the united states and have been identified by major surface protein 1a (msp1a), which varies in sequence and molecular weight due to different numbers of tandem 28- to 29-amino-acid repeats. the present study was undertaken to examine the genetic variations among isolates of a. marginale ...200312682152
antibody prevalence of eight ruminant infectious diseases in california mule and black-tailed deer (odocoileus hemionus).we tested 276 sera from 18 free-ranging black-tailed and mule deer (odocoileus hemionus) herds in california (usa) collected from 1987 to 1991 in five biogeographical habitat types, for antibodies against eight infectious disease agents. overall antibody prevalence was 56% for anaplasma marginale, 31% for borrelia burgdorferi, 16% for bluetongue virus serotype 17, 15% for epizootic hemorrhagic disease virus, 7% for coxiella burnetii and toxoplasma gondii, respectively, and 0% for bovine leukosis ...19948151824
effects and use of a modified live anaplasma marginale vaccine in beef heifers in california.eighty-one 11-month-old, nonpregnant, anaplasma marginale-seronegative beef heifers were allotted to 2 groups for evaluation of a modified live a marginale vaccine (n = 50 for vaccinated group and n = 31 for nonvaccinated group). the vaccine induced a mild form of anaplasmosis, as evidenced by a significant (p less than 0.01) decrease in the packed cell volume (pcv) between days 31 and 46 after vaccination. the lowest pcv was 11%, and 3 heifers had a pcv less than 20%. slight lethargy was eviden ...19836874525
anaplasma marginale infection in deer in the sierra nevada foothill area of california. 19676067991
mass screening of cattle sera against 14 infectious disease agents, using an elisa system for monitoring health in livestock.mass screening elisa methods were developed for testing cattle serum for antibodies against 14 common livestock diseases simultaneously. the absorbance values were transformed to a %elisa (spectrophotometric antibody end point) by a computer interfaced with a microplate reader. a histogram indicating a cutoff point and a report for the veterinarian also was generated. the computer program produced a print-out of the antibody profile for each animal tested, the antibody concentration against each ...19911767993
prevalence of anaplasma marginale infection in california deer as measured by calf inoculation and serologic techniques. 19695278172
the modified card agglutination test: an accurate tool for detecting anaplasmosis in columbian black-tailed deer.inoculation of susceptible calves confirmed that the modified card agglutination test accurately detected the anaplasmosis infection status of each of 35 columbian black-tailed deer (odocoileus hemionus columbianus). anaplasma marginale, and specific antibodies, were demonstrated only in calves which received blood from deer that were positive by the card test. the modified card agglutination testing of deer serum was performed in the manner recommended for testing cattle serum with bovine-origi ...197616498890
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