| cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil. | anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ... | 2007 | 17278086 |
| molecular detection of tick-borne pathogens of the family anaplasmataceae in brazilian brown brocket deer (mazama gouazoubira, fischer, 1814) and marsh deer (blastocerus dichotomus, illiger, 1815). | deer are important natural reservoir hosts of anaplasmataceae. the present study used nested pcr and nucleotide sequencing to evaluate the occurrence of anaplasmataceae species in 23 free-living and six captive specimens of the cervids mazama gouazoubira and blastocerus dichotomus in minas gerais state, brazil. blood samples were tested for the presence of ehrlichia and anaplasma spp. using npcr assays and sequencing of the msp4, msp1 and 16s rrna genes. the identity of each sequence was confirm ... | 2011 | 22136597 |
| genetic diversity and molecular phylogeny of anaplasma marginale isolates from minas gerais, brazil. | anaplasma marginale (rickettsiales: anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world, and many isolates of a. marginale may occur in a given geographic area. phylogenetic relationships have been reported for a. marginale isolates from the us using gene and protein sequences of msp1a and msp4. these studies demonstrated that msp4 sequences, but not msp1a dna or protein sequences, provide phylogeographic information and also that msp1a ... | 2004 | 15135871 |
| ultrastructure of anaplasma marginale with an inclusion appendage, isolated in minas gerais state, brazil. | this is the first report on the occurrence and isolation of a strain of anaplasma marginale with an inclusion appendage in brazil. the inclusion appendage presented longitudinal electron-dense striations and did not originate directly from the body of the rickettsia but from an electron-dense complex located at the junction of the inclusion membrane and inclusion appendage. the inclusion appendage remained in the host cell after the anaplasma inclusion appeared to be leaving the red blood cell. ... | 1997 | 9211652 |
| detection of genetic diversity of anaplasma marginale isolates in minas gerais, brazil. | bovine anaplasmosis, caused by the tick-borne rickettsia anaplasma marginale, is endemic in tropical and subtropical regions of the world and results in economic losses in the cattle industry. major surface proteins (msps) have been used as markers for the genetic characterization of a. marginale strains and demonstrate that many isolates may occur in a given geographic area. however, in brazil, little is known about the genetic diversity of a. marginale isolates within individual herds. this st ... | 2013 | 24252959 |