| use of repetitive dna elements to define genetic relationships among anaplasma marginale isolates. | anaplasma marginale genomic dna was tested for the presence of repetitive extragenic palindromic (rep) and enterobacterial repetitive intergenic consensus (eric)-like sequences in order to evaluate the genetic diversity of multiple a. marginale isolates. a. marginale isolates were obtained from cattle of six different states of brazil, from the us and an anaplasma centrale strain was obtained from uruguay. patterns obtained from a. marginale isolates varied from 14 to 17 fragments by rep-polymer ... | 2001 | 11313126 |
| antigenic characterization of brazilian isolates of anaplasma marginale. | antigenic characterization of anaplasma marginale isolates, by identifying conserved and variable epitopes of major surface proteins (msp), is an important tool for vaccine development against this rickettsia. the b cell epitopes of a. marginale isolates from three microregions of the state of pernambuco and one from the state of mato grosso do sul, brazil, were characterized by indirect fluorescent antibody technique (ifat) and western blot (wb) with 15 monoclonal antibodies (mabs). the epitope ... | 2003 | 12886423 |
| [detection of antibodies against anaplasma sp. in small ruminants from the semi-arid region against pernambuco state, brazil]. | this paper reports the detection of antibodies against anaplasma sp. in goats and sheep from the semi-arid region from pernambuco state, brazil, using an enzyme-linked immunosorbent assay with recombinant msp5 of anaplasma marginale. sera from 243 goats and 68 sheep from ibimirim municipality were analyzed and frequencies of antibodies of 11.93% (29/243) and 16.17% (11/68) were found for goats and sheep, respectively. the epidemiological relevance of the findings was discussed. | 2008 | 18823582 |
| [transcription of genes of membrane proteins of brazilians isolates of anaplasma marginale]. | this work shows the transcription profile of membrane protein genes in three brazilian isolates of anaplasma marginale (rio grande do norte, pernambuco-zona da mata, and pernambuco-sertão). rna was purified from cattle blood experimentally-infected with the three isolates of a. marginale. after reverse transcription, genes omp1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, and 14; opag1-3; virb3, 9, and 10; am097, 197, 254, 854, and 956 were amplified by pcr, with specific primers. transcripts were dete ... | 2007 | 18078602 |