Publications

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[development of schistosoma mansoni strains from belo horizonte, minas gerais, and são josé dos campos (são paulo) in mus musculus]. 19734789390
studies on human anti-rabies immunization in brazil. i--evaluation of the 3 + 1 pre-exposure vaccination schedule under field conditions.the currently used pre-exposure anti-rabies immunization schedule in brazil is the one called 3+1, employing suckling mouse brain vaccine (3 doses on alternate days and the last one on day 30). although satisfactory results were obtained in well controlled experimental groups using this immunization schedule, in our routine practice, vna levels lower than 0.5 iu/ml are frequently found. we studied the pre-exposure 3+1 schedule under field conditions in different cities on the state of são paulo, ...19958599065
incidence of non-01 vibrio cholerae and aeromonas spp. in fresh water in araraquara, brazil.the occurrence of aeromonas spp., vibrio cholerae, and plesiomonas shigelloides in fresh water from various sources in araraquara, state of são paulo, brazil was determined. samples from ten distinct irrigation systems used in vegetable cultivation, from five distinct streams, from two reservoirs, from one artificial lake, and from three distinct springs were analyzed. all isolates were serotyped and tested for hemolysin, cytotoxin, heat-stable (st) and heat-labile (lt) enterotoxins production; ...19989625786
effect of a booster-dose of rabies vaccine on the duration of virus neutralizing antibody titers in bovines.humoral immune response using inactivated rabies vaccine was studied in 35 nelore cross-bred bovines of western region of são paulo state. ninety days after vaccination, 13 (92.8%) animals presented titers > or = 0.5 iu/ml, through mouse neutralization test. after 180 days, 9 (64.3%) sera showed titers > or = 0.5 iu/ml, after 270 days, only one (7.1%) showed a titer of 0.51 iu/ml, and after 360 days, all animals showed titers < 0.5 iu/ml. group of animals receiving booster dose 30 days after vac ...19989662964
occurrence of neospora caninum antibodies in sera from dogs of the city of são paulo, brazil.neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. in order to understand the prevalence of n. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of são paulo, brazil were assayed for antibodies to n. caninum. sera were examined by the neospora agglutination test (nat) using mouse-derived tachyzoites. anti ...200212031819
decreased fertility in mice exposed to environmental air pollution in the city of sao paulo.it has largely been shown that air pollution can affect human health. effects on human fertility have been shown mainly in males by a decrease in semen quality. few studies have focused on the environmental effects on female fertility. the aim of the present study was to analyze the effects of air pollution in the city of sao paulo on mouse female fertility. four groups of female balb/c mice were placed in two chambers 10 days (newborn) or 10 weeks (adults) after birth. mice were maintained in t ...200515820725
toxicity of a cyanobacteria bloom in barra bonita reservoir (middle tietê river, são paulo, brazil).in eutrophic waters during cyanobacterial bloom lysis, a blend of cyanobacterial toxins and other compounds are released into the water, affecting aquatic communities. this research investigated the effect of a simulated cyanobacterial lysis event. for this purpose, intact cells from a natural cyanobacterial bloom from barra bonita reservoir (tietê river basin, brazil) were taken, and the cells were broken by repeated freeze/thaw cycles. the toxicity of the crude cyanobacterial extract was inves ...200615993489
biological and immunological properties of the venom of bothrops alcatraz, an endemic species of pitviper from brazil.bothrops alcatraz is a new pitviper species derived from the bothrops jararaca group, whose natural habitat is situated in alcatrazes archipelago, a group of marine islands near são paulo state coast in brazil. herein, the biological and biochemical properties of venoms of four adult specimens of b. alcatraz were examined comparatively to a reference pool of bothrops jararaca venom. both venoms showed similar activities and electrophoretic patterns, but b. alcatraz venom showed three protein ban ...200516002343
cytotoxicity in the marine dinoflagellate prorocentrum mexicanum from brazil.the microscopic algae in the oceans are crucial food for filter feeding bivalve shellfish (oysters, mussels, scallops, clams, etc.) as well as for the larvae of commercially important crustaceans. some species of microalgae have the capacity to produce potent toxins, such as saxitoxins and ciguatoxins, which may intoxicate humans. among the marine phytoplankton, the dinoflagellates are the main toxin producers. studies on the marine phytoplankton from the são sebastião channel, southeastern coas ...200616458613
evaluation of activated sludge treatment and the efficiency of the disinfection of giardia species cysts and cryptosporidium oocysts by uv at a sludge treatment plant in campinas, south-east brazil.among many waterborne diseases the giardiasis and cryptosporidiosis are of particular public health interest, because giardia cysts and cryptosporidium oocysts can persist for long periods in the environment, and both pathogenic protozoa have been implicated as the cause of many outbreaks of gastroenteritis in the last 25 years. in order to evaluate the efficiency of cysts and oocysts' removal by the activated sludge process, and by uv reactor in inactivating cysts and oocysts in one wastewater ...200617037138
increased levels of air pollution and a decrease in the human and mouse male-to-female ratio in são paulo, brazil.a significant negative association (r(2) = 0.7642; p=.013) between particulate matter and secondary sex ratio was found when evaluating people in the city of são paulo, brazil. an animal model with male mice raised in nonfiltered open-top chambers showed a significant reduction in the secondary sex ratio (p=.041), suggesting that ambient air pollution may interfere with sex distribution by altering the x:y sperm proportion in pollution-exposed males.200717084397
mayaro virus: imported cases of human infection in são paulo state, brazil.mayaro virus (mayv) is an arbovirus (togaviridae: alphavirus) enzootic in tropical south america and maintained in a sylvan cycle involving wild vertebrates and haemagogus mosquitoes. mayv cases occur sporadically in persons with a history of recent activities inside or around forests. this paper reports three cases of mayv fever detected in men infected in camapuã, ms, brazil. serum samples collected at four days and two months after the onset of the symptoms and examined by hemagglutination in ...200717823750
population structure and mouse-virulence of toxoplasma gondii in brazil.recent studies found that isolates of toxoplasma gondii from brazil were biologically and genetically different from those in north america and europe. however, to date only a small number of isolates have been analysed from different animal hosts in brazil. in the present study dna samples of 46 t. gondii isolates from cats in 11 counties in são paulo state, brazil were genetically characterised using 10 pcr restriction fragment length polymorphism markers including sag1, sag2, sag3, btub, gra6 ...200817963770
production of monoclonal antibodies against neisseria meningitidis using popliteal lymph nodes and in vivo/in vitro immunization: prevalence study of new monoclonal antibodies in greater são paulo, brazil.a rapid and efficient method for preparing monoclonal antibody (mab) serotypes using neisseria meningitidis outer membrane were used in balb/c mouse footpads for the immunization. the popliteal lymph nodes were isolated 19 days later for mab-producing hybridomas, from which the mabs against the 37 kda protein were screened. variations in class 2/3 (porb) proteins form the basis for meningococcal serotyping. this is the first report on the preparation of mabs against n. meningitidis that is speci ...200717979546
antigenic and genetic characterization of the first rabies virus isolated from the bat eumops perotis in brazil.although the main transmitters of rabies in brazil are dogs and vampire bats, the role of other species such as insectivorous and frugivorous bats deserves special attention, as the rabies virus has been isolated from 36 bat species. this study describes the first isolation of the rabies virus from the insectivorous bat eumops perotis. the infected animal was found in the city of ribeirão preto, são paulo. the virus was identified by immunofluorescence antibody test (fat) in central nervous syst ...200818488088
isolation of toxoplasma gondii from capybaras (hydrochaeris hydrochaeris) from são paulo state, brazil.the capybara (hydrochaeris hydrochaeris) is a large rodent used for human consumption in certain areas of south america. in the present study, viable toxoplasma gondii was isolated for the first time from this host. antibodies to t. gondii were assayed in the sera of 64 capybaras from 6 counties of são paulo state, brazil, using the modified agglutination test (mat, > or =1:25 dilution) and the indirect fluorescent antibody test (ifat, > or =1:16 dilution), and antibodies were found in 48 (75%) ...200818576842
seroprevalence and isolation of toxoplasma gondii from sheep from são paulo state, brazil.sheep are important in the epidemiology of toxoplasma gondii infection but little is known of ovine toxoplasmosis in brazil. antibodies to t. gondii were assayed in sera of 495 sheep from 36 counties of são paulo state, brazil, using the modified agglutination test (mat titer > or =1:25) and found in 120 (24.2%). samples of brain, heart, and diaphragm of 82 seropositive sheep were pooled, digested in pepsin, and bioassayed in mice. toxoplasma gondii was isolated from tissue homogenates of 16 she ...200818576886
chronic exposure to ambient levels of urban particles affects mouse lung development.chronic exposure to air pollution has been associated with adverse effects on children's lung growth.200818596224
[epidemiological profile of rabies in the northwestern region of são paulo state, from 1993 to 2007].the epidemiology of animal rabies in the region of araçatuba, in the northwest of são paulo state, from 1993 to 2007, is described according to the results from diagnoses made at laboratories in the region, using the fluorescent antibody and mouse inoculation tests. out of 10,579 samples analyzed, 4.9% were positive (518/10,579). dogs accounted for 67% of the cases (346/518) and these occurred between 1993 and 1997. among the other positive samples, 16% (84/518) were in cattle and 9.7% (50/518) ...200919287928
genetic diversity among capybara (hydrochaeris hydrochaeris) isolates of toxoplasma gondii from brazil.recent studies indicate that toxoplasma gondii isolates of many domestic hosts from brazil are genetically and biologically different from t. gondii isolates from usa and europe. however, little is known about genetics of t. gondii isolates from wild mammals in brazil. in this study, genotypes of 36 t. gondii isolates from capybaras (hydrochaeris hydrochaeris) from six counties in são paulo state, brazil, were determined. sixteen genotypes were identified using 11 genetic markers including sag1, ...200919375864
genetic diversity among toxoplasma gondii isolates of small ruminants from brazil: novel genotypes revealed.recent studies indicated that toxoplasma gondii isolates of many domestic animal hosts from brazil are genetically and biologically different from those in usa and europe. despite of high pathogenicity of this parasite to small ruminants, the epidemiology and genetic diversity of t. gondii in these animals are not well understood in brazil. in this study, a total of 28 t. gondii samples (16 isolates from sheep in são paulo state, and 12 isolates from goats in the states of são paulo and rio gran ...201020236768
detection of toxoplasma gondii oocysts in environmental samples from public schools.the number of toxoplasma gondii oocysts that can be found in random environmental samples is probably low; in addition, these cysts may be confused with hammondia spp. and neospora spp. oocysts. the aim of the present work was to evaluate the presence of t. gondii oocysts in the soil of public elementary schools in the northwest area of the state of são paulo, brazil using mouse bioassays. a comparison was made between the different available bioassay techniques, such as squash, histopathology, ...201020347524
highly toxic microcystis aeruginosa strain, isolated from são paulo-brazil, produce hepatotoxins and paralytic shellfish poison neurotoxins.while evaluating several laboratory-cultured cyanobacteria strains for the presence of paralytic shellfish poison neurotoxins, the hydrophilic extract of microcystis aeruginosa strain spc777--isolated from billings's reservoir, são paulo, brazil--was found to exhibit lethal neurotoxic effect in mouse bioassay. the in vivo test showed symptoms that unambiguously were those produced by psp. in order to identify the presence of neurotoxins, cells were lyophilized, and the extracts were analyzed by ...201120376712
chlamydophila psittaci and toxoplasma gondii infection in pigeons (columba livia) from são paulo state, brazil.pigeons (columba livia) cohabit with humans in urban and rural areas, representing a public health problem since microorganisms are transmitted through the inhalation of dust from their dry feces (chlamydiosis) and through ingestion of their undercooked or poorly refrigerated meat (toxoplasmosis). this study aimed to evaluate the presence of chlamydophila psittaci and toxoplasma gondii in pigeons from four cities in são paulo state, brazil. c. psittaci was evaluated through hemi-nested polymeras ...201021055877
characterization of rabies virus isolated from a colony of eptesicus furinalis bats in brazil.some bat species have adapted to the expanding human population by acquiring the ability to roost in urban buildings, increasing the exposure risk for people and domestic animals, and consequently, the likelihood of transmitting rabies. three dead bats were found in the yard of a house in an urban area of jundiaí city in the state of são paulo in southeast brazil. two of the three bats tested positive for rabies, using fluorescent antibody and mouse inoculation techniques. a large colony of epte ...201121412617
rabies diagnosis and serology in bats from the state of são paulo, brazil.bats are one of the most important reservoirs and vectors of the rabies virus in the world.201121468481
first report of rabies infection in bats, molossus molossus, molossops neglectus and myotis riparius in the city of são paulo, state of são paulo, southeastern brazil.this paper presents the first report of rabies in three bat species, molossus molossus, molossops neglectus and myotis riparius in the city of são paulo, brazil.201121503551
[the bats and rabies in the western region of the state of são paulo, brazil.]introduction: the polo da alta sorocabana laboratory in presidente prudente, sp, in partnership with other research institutions, conducted studies related to bats from the western region of the state of sao paulo, brazil. thus, certain situations were investigated, including: a) isolation of the rabies virus from 2006 to 2008; b) identification of respective antigenic variants; and c) characterization of daytime shelters of desmodus rotundus vampire bats. methods: samples for examination origin ...201121556491
a survey of neospora caninum and toxoplasma gondii infection in urban rodents from brazil.abstract toxoplasma gondii is a protozoan parasite that infects humans and other warm blooded animals; it uses feral and domestic cats as the definitive hosts. neospora caninum is a protozoan parasite of animals whose life cycle is very similar to t. gondii, but uses canids as definitive hosts. small rodents play an important role in the life cycle of t. gondii and a few findings indicated that they may be natural intermediate hosts for n. caninum. the present study was aimed at identifying infe ...201121790367
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