| calibrated serological techniques demonstrate significant different serum response rates to an oral killed cholera vaccine between swedish and nicaraguan children. | serum responses to oral cholera vaccines were assessed in three paediatric vaccine trials, two in león, nicaragua and one in stockholm, sweden. a calibrated anti-cholera toxin b subunit (ctb) iga elisa was used together with an assay for vibriocidal antibodies. swedish children had lower pre-vaccination levels of antibody, but serum responses were more pronounced in swedish children than in nicaraguan children. post-vaccination levels of anti-toxin antibody were generally above those found after ... | 2002 | 12443672 |
| detection and quantification of vibrio populations using denaturant gradient gel electrophoresis. | bacteria affiliated with the genus vibrio are endemic in marine and estuarine ecosystems and are also found in many freshwater environments. vibrios can enter viable but non-culturable states and since many species are pathogenic, there is a great need for culture-independent methods that identify and quantify multiple vibrio populations. we adopted vibrio-specific 16s rrna-directed primers and a competitive pcr protocol (qc-pcr; [thompson, j.r., randa, m.a., marcelino, l.a., tomita-mitchell, a. ... | 2006 | 16730823 |