restriction fragment length polymorphism analysis of vibrio cholerae strains associated with a cholera outbreak in hong kong. | we studied vibrio cholerae el tor isolates associated with an outbreak of cholera among vietnamese refugees interned in hong kong. the restriction fragment length polymorphism of the enterotoxin gene was used as an epidemiological marker. all outbreak strains were indistinguishable. they were distinct from strains isolated in hong kong prior to the outbreak. | 1991 | 1676035 |
molecular epidemiology of vibrio cholerae in hong kong. | we studied restriction fragment length polymorphism of the enterotoxin genes of isolates of vibrio cholerae el tor, indistinguishable by bacteriophage typing, which were collected in hong kong since 1978. using this approach, we could distinguish indigenous and exogenous strains obtained from different sources and epidemiological settings. | 1989 | 2570082 |
[the el-tor cholera pandemic in the years 1961-1968]. | | 1973 | 4279652 |
survey of intestinal pathogens from immigrant children. | four thousand immigrant schoolchildren were screened by using a concentration method on a single faecal specimen for intestinal helminths; 1,468 (37%) were positive. carriage rates were highest (51%) in children from the west indies, in whom trichuris (75% of carriers) and ascaris (40% of carriers) predominated; carriage of two or more species was common (36% of carriers). carriage of worms was low in children from kenya (16%) and uganda (7%). children from south-east asia, predominantly from in ... | 1972 | 5013832 |
outbreak of vibrio cholerae 01 in hong kong related to contaminated fish tank water. | an outbreak of 12 cholera cases, caused by vibrio cholerae eltor inaba, occurred in hong kong during a three week period in june-july 1994. only adults of both sexes were affected. epidemiological investigations showed linkage in all cases with consumption of seafood, including shellfish, mantis shrimps and crabs. microbiological findings demonstrated that contaminated seawater in fish tanks used for keeping alive these seafoods is the most likely vehicle of transmission. aggressive control meas ... | 1995 | 7480605 |
vibrio cholerae o139 synonym bengal in hong kong. | | 1994 | 7811886 |
resurgence of cholera in hong kong. | cholera is one of the three diseases subject to the international health regulations. after a period of over 30 years, the seventh pandemic of cholera, which started in south east asia in 1961, still shows no sign of a decline. on the contrary, it has increased its severity and invaded many other countries in africa and latin america. in the last two years, there has been a recrudescence of the disease in south east asia and western pacific regions. the discovery of a new strain of vibrio choler ... | 1996 | 8760949 |
ofloxacin-resistant vibrio cholerae o139 in hong kong. | | 1999 | 10458975 |
peak occurrences of ciguatera fish poisoning precede cholera outbreaks in hong kong. | occurrences of ciguatera fish poisoning (cfp) and vibrio cholerae infected patients in hong kong were reviewed for the 13-year period 1989-2001. peak activity of cfp preceded peak activity of cholera in nine of the years except in 4 years (1990, 1991, 1992, 1996) where it was observed that the total number of cholera cases were all less than or equal to five per year (p < 0.05). average time interval was 2.4 months between peaks of cfp and vibrio cholerae outbreaks. findings suggested that the f ... | 2003 | 12948360 |
molecular subtyping of vibrio cholerae o1 and o139 by pulsed-field gel electrophoresis in hong kong: correlation with epidemiological events from 1994 to 2002. | two hundred twenty isolates of vibrio cholerae o1 and o139 collected from 1994 to 2002 in hong kong were analyzed by pulsed-field gel electrophoresis (pfge). chromosomal dnas from all v. cholerae isolates in agarose plugs were digested with the restriction enzyme noti, resulting in 20 to 27 bands. sixty distinctive pfge patterns in the range of 10 to 300 kb were noted among 213 isolates typeable by pfge. by comparing the common pfge patterns obtained from four well-defined outbreaks of v. choler ... | 2003 | 14532174 |
influence of temperature, salinity and ph on the growth of environmental aeromonas and vibrio species isolated from mai po and the inner deep bay nature reserve ramsar site of hong kong. | four environmental bacterial isolates including aeromonas hydrophila mp-3, a. salmonicida mp-4, vibrio vulnificus mp-2 and v. cholerae mp-1 isolated from sediment and water of mai po nature reserve of hong kong were examined for their responses to temperature, ph and salinity under laboratory conditions in this study. v. cholerae mp-1 was found to resist vibriostatic agent o/129 at concentration of 10 microg/ml. in addition, bacterial growth under test conditions was measured and the results wer ... | 2005 | 15678554 |
development and validation of a pulsenet standardized pulsed-field gel electrophoresis protocol for subtyping of vibrio cholerae. | pulsenet is a network that utilizes standardized pulsed-field gel electrophoresis (pfge) protocols with the purpose of conducting laboratory-based surveillance of foodborne pathogens. pulsenet standardized pfge protocols are subject to rigorous testing during the developmental phase and careful evaluation during a validation process assessing its robustness and reproducibility in different laboratories. here we describe the development and validation of a rapid pfge protocol for subtyping vibrio ... | 2006 | 16602979 |
antibiotic resistance and plasmid profile of environmental isolates of vibrio species from mai po nature reserve, hong kong. | in this study, three environmental isolates of vibrio species were isolated from water and sediment samples of mai po nature reserve, hong kong sar. they were identified to be vibrio cholerae mp-1, vibrio aestuarianus mp-2 and vibrio vulnificus mp-3 by both biochemical test and subsequent full-length 16s rdna sequencing. both disc diffusion susceptibility test and microplate dilution technique were used to investigate the antibiotic resistance profile of these three bacteria. results from both a ... | 2006 | 16680524 |
rapid detection of food-borne pathogens in clinical specimens, food and environmental samples. | 1. pcr and rt-pcr methods for 5-hour and 3-hour detection, respectively, of salmonellae and vibrio cholerae in stool, food and environmental water samples have been developed. 2. such methods can be used in routine laboratories for rapid detection of salmonellae and v cholerae and are essential for infection control purposes. | 2009 | 19258630 |
high incidence of plasmids in marine vibrio species isolated from mai po nature reserve of hong kong. | mai po nature reserve is the largest mangrove ecosystem and the most polluted coastal water body in hong kong. plasmids screening of 100 vibrio isolates randomly showed 45 % of them contained 1-3 plasmids. these plasmid(s)-bearing isolates could be divided into 12 groups based on their plasmid profiles. phylogenetic analysis of the partial 16s rrna gene sequences confirmed that all plasmid(s)-bearing isolates belonged to vibrio cholerae. full dna sequences of the plasmids in groups i (pvcg1.1 an ... | 2012 | 22684730 |