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molecular characterization of an outbreak of respiratory syncytial virus (subgroup a) in havana, cuba, by monoclonal antibodies and restriction mapping (n gene).twenty-one respiratory syncytial virus (rsv) strains isolated from one outbreak in havana, cuba (1994 to 1995), were analyzed to determine their relatedness. all isolated strains were classified as subgroup a by monoclonal antibodies. of 21 rsv strains examined, 20 were classified as having restriction pattern np4 and only 1 was classified as having restriction pattern np5.19979302221
unusual antigenic and genetic characteristics of human respiratory syncytial viruses isolated in cuba.the g protein of 23 strains of human respiratory syncytial virus isolated in havana, cuba, between october 1994 and january 1995 was analyzed at the antigenic and genetic level. all viruses reacted with 10 of 11 antibodies specific for the long strain. moreover, the g protein gene of the cuban isolates had only five nucleotide differences from the sequence of the long gene. the homogeneity of the cuban isolates and their resemblance to an ancient strain, such as long, are at odds with previous f ...19989696858
antigenic and genetic characterization of twenty-six strains of human respiratory syncytial virus (subgroup a) isolated during three consecutive outbreaks in havana city, cuba.twenty-six human respiratory syncytial virus strains (subgroup a) isolated from three outbreaks in havana city during the period 1994/95, 1995/96 and 1996/97 were analyzed to determine their antigenic and genetic relationships. analyses were performed by monoclonal antibodies and restriction mapping (n gene) following amplification of the select region of the virus genome by polymerase chain reaction. all isolated strains were classified as subgroup a by monoclonal antibodies and they showed a r ...199910446003
[subgroups classification of strains of the respiratory syncytial virus isolated in an outbreak in ciudad de la havana].a high number of acute respiratory diseases was detected among children under one year admitted in a hospital of havana city. 25 respiratory syncytial virus strains were obtained from 93 patients studied. viral isolations were multiplied in hep-2 cells and after observing a cytopathic effect of 80%, they were classified into subgroups by the indirect immunofluorescence technique, using anti-protein g antibodies from the respiratory syncytial virus. all the samples studied were classified within ...19969768286
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