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[control of arboviral encephalitis in china (author's transl)].japanese b encephalitis is the most important and widespread arbovirus disease in china. except tibet and sinjiang autonomous regions, it occured in every province. jbe virus were isolated from six species of culex, three species of aedes, one species of anopheles and lasiohelea. the main vector of jbe virus in china is culex tritaeniorhynchus. the main spreading reservoir host, no doubt, is the pig, but the persistant reservoir is not clear yet. vaccination of the children under ten years is an ...19806255282
trichinellosis in china: epidemiology and control.the first outbreak of human trichinellosis in china, according to official national publication, was registered in tibet in 1964. since then, more than 500 outbreaks, numbering 25161 cases with 240 deaths, have been recorded. however, this reported quantity is probably highly underestimated because adequate diagnostic techniques might not have been available in the whole area. moreover, infection in animals is widespread over china, with the prevalence of pig trichinellosis being as high as 4% i ...200212482541
sequencing and analysis for the full-length genome rna of foot-and-mouth disease virus china/99.the complete nucleotide sequence of genomic rna of foot and mouth disease virus (fmdv) strain china/99 from infected bovine tongue epithelium is presented. the nucleotide sequence extending from the 5' end of the genomic rna to the 5' end of poly (a) tail contains 8173 nucleotides (nt). its open reading frame, which encodes a single polypeptide of 2332 amino acids, encompasses 6999 nt starting from the initiation codon aug and terminating at the uaa codon 93 bases upstream from the 5' end of pol ...200415382679
distribution of escherichia coli f4 adhesion phenotypes in pigs of 15 chinese and western breeds and a white durocxerhualian intercross.diarrhoea in newborn and weaned piglets is mainly caused by enterotoxigenic escherichia coli (etec) with fimbriae f4. to investigate the prevalence of resistance to three fimbrial strains, f4ab, f4ac and f4ad, among chinese indigenous pigs and western commercial pigs introduced into china, we determined the etec f4 adhesion phenotypes in 292 pure-bred piglets from three western commercial breeds and 12 chinese indigenous breeds, and a total of 1093 adult pigs in a white durocxerhualian intercros ...200919574416
mosquitoes and mosquito-borne arboviruses in the qinghai-tibet plateau--focused on the qinghai area, china.an investigation was conducted to identify the distribution of mosquitoes and mosquito-borne arboviruses in the qinghai-tibet plateau, china from july to august in 2007. a total of 8,147 mosquitoes representing six species from three genera (aedes, culex, and anopheles) were collected in three locations (geermu city, altitude of 2,780 m; xining city, 2,200 m; minhe county, 1,700 m). six virus isolates were obtained including tahyna virus (tahv), liaoning virus, and culex pipiens pallens densovir ...201020348523
genetic characterization of the cell-adapted panasia strain of foot-and-mouth disease virus o/fujian/cha/5/99 isolated from swine.according to office international des epizooties (oie) bulletin, the panasia strain of foot-and-mouth disease virus (fmdv) was invaded into the people's republic of china in may 1999. it was confirmed that the outbreaks occurred in tibet, hainan and fujian provinces. in total, 1280 susceptible animals (68 cattle, 1212 swine) were destroyed for the epidemic control.to investigate the distinct biological properties, we performed plaque assay, estimated the pathogenicity in suckling mice and determ ...201020807416
japanese encephalitis, tibet, china.to the editor: tibet is located in the qinghai-tibet plateau of western people's republic of china and has been internationally recognized as a japanese encephalitis (je)-nonendemic area because the average altitude is thought to be too high to facilitate the cycle of japanese encephalitis virus (jev) between mosquitoes and vertebrates (1,2). in addition, je is a reportable infectious disease in china, and no clinically confirmed case has been reported in tibet since establishment of a national ...201121529419
[effects of multi-genes for reproductive traits in tibet pig].tibet pig is a unique native breed in the plateau of china, which has good adaptation to the harsh climate of high land and resistance to diseases and crude feeding. however, its reproductive rate is low. the objectives of this study were to search for the polymorphisms of estrogen receptor (esr), follicle-stimulating hormone (fshb), prolactin receptor (prlr), and retinol binding protein 4 (rbp4) in tibet pig and to analyze the effects of these variants and their combination genotypes on reprodu ...201020466637
[mitochondrial dna d-loop variation types in tibet mini-pigs in association with the blood parameters].to analyze the mitochondrial dna (mtdna) d-loop region sequence variation in tibet mini-pigs in relation to the blood parameters and provide the molecular genetic basis for developing new species of laboratory animals.200919726312
molecular cloning, tissue distribution and ontogenetic expression of the amino acid transporter b(0,+) cdna in the small intestine of tibetan suckling piglets.the small intestine is the main absorption place of peptides and free amino acids in mammals. the amino acid transporter system b(0,+) mediates apical uptake of basic amino acids, especially lysine, arginine and cysteine. the aim of the current study was to clone tibetan porcine amino acid transporter b(0,+)at (slc7a9) for comparing the sequences of tibetan and common (sus scrofa) pigs, and investigating the tissue distribution and ontogenetic expression in the small intestine of tibetan sucklin ...200919501668
[effect of alexandrite laser treatment for hair removal in tibet mini-pigs].to observe the histological and ultrastructural changes of the skin and hair follicles following hair removal by alexandrite laser in tibet mini-pigs.200919403398
[polymorphisms of three major genes for reproductive traits in tibet pigs].tibet pig is a special chinese indigenous pig breed, which could survive in high-altitude areas of tibet. in the present study, the genotypes of esr, fshbeta, and prlr gene, the three known major genes of reproductive traits, were identified in 202 tibet pig individuals. the results showed that the predominant genotypes of esr, fshbeta, and prlr gene were bb, ab and aa, respectively. the genotype frequencies of the three genes were in hardy-weinberg equilibrium. the frequencies of desired allele ...200717681921
porcine eperythrozoonosis in china.eperythrozoonosis of swine (also designated as porcine mycoplasmosis) is a disease of swine under stress, expressed as a febrile condition with development of an acute ictero-anemia. it is caused by eperythrozoon suis and usually causes a subclinical infection with a latent carrier state that persists for extended periods. in china, this disease has gradually developed as an important intercurrent disease and an emerging swine disease that, in recent years, has spread throughout all provinces ex ...200617135527
the epidemiology of human trichinellosis in china during 2000-2003.the endemic foci of trichinellosis continue to be mainly located in the southwestern, the central and northeastern china. the seroepidemiological surveys of t. spiralis infection in humans were carried out in six provinces or municipals (p/m) of china during 2000-2003, the overall seroprevalence was 3.57%. from 2000 to 2003, 17 outbreaks of human trichinellosis, with 828 cases and 11 deaths, were recorded in 8 p/a of china. all of 11 deaths occurred in the southwestern china (1 case in sichuan, ...200616414006
molecular cloning and expression of il2 cdna from the tibet pig.interleukin-2 is a vital cytokine secreted by activated t lymphocytes, and plays important role in the regulation of cellular and humoral immunity of animals. in our experiment, il2 cdna of the tibet pig was first cloned by rt-pcr from cona-stimulated lymphocytes in the blood and subcloned into pmd-18 t vector, which then was identified with endonuclease restriction. the sequencing result showed that tibet pig il-2 (tpil-2) cdna was 503 bp long (orf was 465 bp) (genbank accession number: ay 2940 ...200516195934
[the genetic diversity of the second exon about sla-dqb in some chinese native pigs].polymorphism of the second exon about sla-dqb genes in some chinese native pigs was investigated employing pcr-sscp analysis and dna cloning sequencing. combined with the sequences in genbank, 73 new alleles were gained, among them function alleles of sla-dqb were sixty-eight, five alleles of pseudogene (sla-dxb) were gained. there were disequilibrium distribution of the alleles in all breeds, the same allele was shared by most breeds. c08 allele existed in 11 breeds of six groups and in the wil ...200515843340
mitochondrial dna evidence indicates the local origin of domestic pigs in the upstream region of the yangtze river.previous studies have indicated two main domestic pig dispersal routes in east asia: one is from the mekong region, through the upstream region of the yangtze river (uryz) to the middle and upstream regions of the yellow river, the other is from the middle and downstream regions of the yangtze river to the downstream region of the yellow river, and then to northeast china. the uryz was regarded as a passageway of the former dispersal route; however, this assumption remains to be further investig ...201223272130
the local origin of the tibetan pig and additional insights into the origin of asian pigs.the domestic pig currently indigenous to the tibetan highlands is supposed to have been introduced during a continuous period of colonization by the ancestors of modern tibetans. however, there is no direct genetic evidence of either the local origin or exotic migration of the tibetan pig.201122163285
the epidemiology of human trichinellosis in china during 2004-2009.the endemic foci of human trichinellosis are mainly located in southwestern china. seroepidemiological surveys of trichinella spiralis infection in humans were carried out in 10 out of 34 provinces/autonomous regions/municipals (p/a/m) of china during 2004-2009. the overall seroprevalence was 3.19% (3198/100,282). the highest seroprevalences were mainly located in western china: 8.43% in yunnan, 6.37% in inner mongolia and 5.35% in sichuan. the seroprevalence of trichinella infection in humans w ...201121324302
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