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first molecular identification of sarcocystis miescheriana (protozoa, apicomplexa) from wild boar (sus scrofa) in iran.sarcocystis isolate obtained from the thigh muscle of a wild boar (sus scrofa), captured from gilan province, northern iran, was subjected to molecular analysis. genomic dna was obtained using a dna extraction tissue kit and polymerase chain reaction (pcr) for amplification of the 18s ribosomal dna region yielded an 842 bp dna band on agarose gel. analysis of dna sequencing by blast confirmed the isolate as sarcocystis miescheriana and the sequence was deposited in genbank by accession no. gu395 ...201021095184
the first occurrence of trichinella murrelli in wild boar in iran and a review of iranian trichinellosis.trichinella larvae isolated from the thigh muscle of a wild boar, sus scrofa, captured from gilan province, northern iran, was processed for dna analysis. polymerase chain reaction (pcr) for amplification of the 5s rdna fragment demonstrated a 700 bp band on agarose gel. analysis of dna sequencing by blast confirmed the isolate as t. murrelli. this report constitutes the first recorded occurrence of t. murrelli in asia, and also the first occurrence in a wild boar host.200919531274
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