| polymerase chain reaction monitoring of transmission of onchocerca volvulus in two endemic states in mexico. | to investigate the impact of mass ivermectin treatments in mexico on onchocerca volvulus transmission, entomologic surveys were carried out in the two endemic states of oaxaca and chiapas. the data suggest that substantial progress towards the goal of elimination has been made. a comparison pre- and post-ivermectin data from a community in southern chiapas showed a 97% decrease in seasonal transmission potential, but some level of polymerase chain reaction positivity was still detectable. in oth ... | 2004 | 14971696 |
| prevalence of antibodies to onchocerca volvulus in residents of oaxaca, mexico, treated for 10 years with ivermectin. | studies to determine the prevalence of antibodies to onchocerca volvulus, prior to and after actions carried out to interrupt transmission, are scarce in mexico. here we report the prevalence of immunoglobulin g (igg) and igg4 antibodies in an enzyme-linked immunosorbent assay (elisa) against a crude extract of o. volvulus adult worm in serum samples from persons under noninterrupted biannual treatment with ivermectin in areas of onchocercosis endemicity in mexico. to perform the prevalence stud ... | 2005 | 15642982 |
| molecular studies of onchocerca volvulus isolates from mexico. | dna from onchocerca volvulus from oaxaca and chiapas, mexico were used as templates to amplify members of the o-150 onchocerca specific repeat sequence family. the resulting pcr amplicons all hybridized with ovs2, an oligonucleotide that has been previously shown to recognize amplicons derived from o. volvulus with 100% sensitivity. however, when pcr products amplified from the o. volvulus specific plasmid povs134 were used as a probe, most samples did not hybridize. similarly, when pcr products ... | 2006 | 15919244 |
| large-scale entomologic assessment of onchocerca volvulus transmission by poolscreen pcr in mexico. | to study the impact of mass mectizan treatment on onchocerca volvulus transmission in mexico, entomological surveys were carried out in the endemic foci of oaxaca, southern chiapas, and northern chiapas. collected flies were screened by polymerase chain reaction (pcr) for o. volvulus parasites. the prevalence of infected and infective flies was estimated using the poolscreen algorithm and with a novel probability-based method. o. volvulus infective larvae were not detected in flies from 6/13 com ... | 2006 | 16760515 |
| evidence for suppression of onchocerca volvulus transmission in the oaxaca focus in mexico. | entomologic and serologic surveys were performed in four sentinel communities in the oaxaca focus in southern mexico to assess the level of transmission and exposure incidence to onchocerca volvulus. all communities have been receiving ivermectin mass treatment twice per year since 1997. in one community, parasite dna was detected by polymerase chain reaction-enzyme-linked immunosorbent assay in 2004 in one pool of 50 vector heads of 170 such pools (8,500 flies) examined, which indicated an esti ... | 2008 | 18187798 |
| interruption of transmission of onchocerca volvulus in the oaxaca focus, mexico. | all endemic communities of the oaxaca focus of onchocerciasis in southern mexico have been treated annually or semi-annually with ivermectin since 1994. in-depth epidemiologic assessments were performed in communities during 2007 and 2008. none of the 52,632 simulium ochraceum s.l. collected in four sentinel communities was found to contain parasite dna when tested by polymerase chain reaction-enzyme-linked immunosorbent assay (pcr-elisa), resulting in an upper bound of the infection rate in the ... | 2010 | 20595472 |