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serologic testing for human granulocytic ehrlichiosis at a national referral center.an indirect immunofluorescence assay (ifa) was used to identify patients with antibodies reactive to the human granulocytic ehrlichiosis (hge) agent. serum samples collected from clinically ill individuals were submitted to the centers for disease control and prevention by physicians via state health departments from throughout the united states and tested against a panel of ehrlichial and rickettsial pathogens. antibodies reactive to the hge agent were detected in 142 (8.9%) of 1,602 individual ...19999986812
ticks and tick-borne pathogens and putative symbionts of black bears (ursus americanus floridanus) from georgia and florida.ticks were collected from 38 black bears (ursus americanus floridanus) from northwestern florida (n = 18) from 2003 to 2005 and southern georgia (n = 20) in 2006. five species (amblyomma americanum, a. maculatum, dermacentor variabilis, ixodes scapularis, and i. affinis) were collected from florida bears, and 4 species (a. americanum, a. maculatum, d. variabilis, i. scapularis) were collected from bears in georgia. ixodes scapularis was the most frequently collected tick, followed by d. variabil ...200919413369
molecular detection of bacteria in the families rickettsiaceae and anaplasmataceae in northern crested caracaras (caracara cheriway).bacterial pathogens of the families anaplasmataceae and rickettsiaceae are often spread to humans or other animals from bites from infected arthropod hosts. recently, an increasing number of studies have implicated migratory birds in the circulation of these pathogens through the spread of arthropod vectors. however, few studies have examined the potential for resident bird populations to serve as reservoirs for these zoonoses. in this study, we used nested pcrs of the groesl and 17 kda genes to ...201626837860
the pcs20 pcr assay for ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the united states of america.white-tailed deer are susceptible to heartwater (ehrlichia [cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the united states. it is vital, therefore, to validate a highly specific and sensitive detection method for e. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as ehrlichia chaffeensis, anaplasma (ehrlichia) phagocytophilum (hge agent) and ...200415373331
characterization of the major antigenic protein 2 of ehrlichia canis and ehrlichia chaffeensis and its application for serodiagnosis of ehrlichiosis.canine monocytic ehrlichiosis, caused by ehrlichia canis or ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. coinfections with these agents may be common in certain areas of endemicity. currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. previously, we developed two indirect enzyme-linked immunosorbent assays (elisas) using the major antigenic protein 2 (map2) of e. chaffeensis and e. canis. in this study, ...200312853379
prevalence of tick-borne pathogens in host-seeking amblyomma americanum (acari: ixodidae) and odocoileus virginianus (artiodactyla: cervidae) in florida.amblyomma americanum (l.), the lone star tick, is an aggressive tick that is expanding its geographic range within the united states. this tick is the vector for the human and veterinary pathogens ehrlichia chaffeensis and ehrlichia ewingii and is associated with other microbes of unspecified pathogenicity including rickettsia amblyommii, panola mountain ehrlichia, and borrelia lonestari in florida, there has been sparse contemporary data on the prevalence of these organisms in host-seeking lone ...201627117680
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