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coexistence of antibodies to tick-borne pathogens of babesiosis, ehrlichiosis, and lyme borreliosis in human sera.serum specimens from persons with or without lyme borreliosis were analyzed by indirect fluorescent antibody staining methods for total immunoglobulins to babesia microti, ehrlichia chaffeensis (arkansas strain), and ehrlichia equi (mrk strain). there was serologic evidence of human exposure to multiple tick-borne agents in 15 (6.6%) of 227 serum samples obtained in connecticut and minnesota. of these, 10 serum samples were from connecticut patients who had erythema migrans and antibodies to bor ...19958576376
short report: geographic distribution of different genetic types of ehrlichia chaffeensis.the 120-kd protein gene of ehrlichia chaffeensis was used to characterize ehrlichial dna from seven pools of adult amblyomma americanum ticks. ticks from missouri, kentucky, and north carolina contained e. chaffeensis dna of the arkansas strain genotype. ticks from north carolina also contained ehrlichiae of the sapulpa strain genotype, originally identified in oklahoma.19979230803
antibodies to multiple tick-borne pathogens of babesiosis, ehrlichiosis, and lyme borreliosis in white-footed mice.serum samples from peromyscus leucopus (white-footed mouse), collected in connecticut (usa) in 1983, 1985, and during 1990 to 1993, were analyzed by an enzyme-linked immunosorbent assay (elisa) or indirect fluorescent antibody (ifa) staining methods for antibodies to borrelia burgdorferi (strain 2591), babesia microti, ehrlichia chaffeensis (arkansas strain), and ehrlichia equi (mrk strain). of the 294 serum samples tested, 160 (54%) contained immunoglobulins to one or more of these pathogens. t ...19979249691
serological responses to ehrlichia equi, ehrlichia chaffeensis, and borrelia burgdorferi in patients from new york state.serological testing at the new york state department of health for human granulocytic ehrlichiosis in the residents of westchester county, n.y., was performed with specimens from 176 patients by the indirect fluorescent-antibody (ifa) technique with ehrlichia equi mrk-infected neutrophils. to understand whether human monocytotropic ehrlichiosis also occurs in this northeastern geographic region, specimens were also tested for antibodies to ehrlichia chaffeensis arkansas. screening tests and immu ...19979276387
gastrointestinal and hepatic manifestations of human ehrlichiosis: 8 cases and a review of the literature.ehrlichiosis has emerged as an increasingly recognized tick-borne rickettsial disease. it can affect multiple organs including the gastrointestinal tract and liver. signs and symptoms include abdominal pain, nausea, vomiting, diarrhea, jaundice, and hepatosplenomegaly. patients commonly have elevated liver transaminases early in the course of illness as well as leukopenia and thrombocytopenia. if not diagnosed and treated in a timely fashion, ehrlichiosis can progress to multiorgan failure.199910436355
characterization of the major antigenic protein 2 of ehrlichia canis and ehrlichia chaffeensis and its application for serodiagnosis of ehrlichiosis.canine monocytic ehrlichiosis, caused by ehrlichia canis or ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. coinfections with these agents may be common in certain areas of endemicity. currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. previously, we developed two indirect enzyme-linked immunosorbent assays (elisas) using the major antigenic protein 2 (map2) of e. chaffeensis and e. canis. in this study, ...200312853379
natural and experimental infection of white-tailed deer (odocoileus virginianus) from the united states with an ehrlichia sp. closely related to ehrlichia ruminantium.an ehrlichia sp. (panola mountain [pm] ehrlichia sp.) closely related to ehrlichia ruminantium was recently detected in a domestic goat experimentally infested with lone star ticks (lsts, amblyomma americanum) collected from georgia, usa. the infected goat exhibited pyrexia and mild clinical pathologic abnormalities consistent with ehrlichiosis. at least two other ehrlichia species (ehrlichia chaffeensis and ehrlichia ewingii) are maintained in nature by a cycle involving lsts as the primary vec ...200818436670
strains of ehrlichia chaffeensis in southern indiana, kentucky, mississippi, and north carolina.rates of infection of amblyomma americanum (l.) by ehrlichia chaffeensis were compared in 100 ticks collected from sites in each of four states: indiana, north carolina, kentucky, and mississippi. the overall infection rates were similar among sites, ranging from 1 to 4%. because pathogenic differences may exist between e. chaffeensis strains, nested polymerase chain reaction (pcr) amplification of the variable-length pcr target (vlpt), and sequencing of the amplicons were performed to different ...200919960699
ehrlichia ewingii infection and exposure rates in dogs from the southcentral united states.we used pcr and a novel serologic assay to determine infection and exposure rates to ehrlichia ewingii in dogs from an area of northeast oklahoma and northwest arkansas where amblyomma americanum ticks are abundant. of 143 dogs assayed, 13 (9.1%) harbored e. ewingii by pcr and 64 (44.8%) had antibodies to e. ewingii detected using a peptide-based microtiter plate elisa. dogs were more likely (p=0.001) to be positive by pcr if sampled in august (30.8%) but no association was found between seropos ...201020541322
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