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yersinia enterocolitica and related species isolated in san luis, argentina, 1989-1993. 19958833797
pulsed field, pcr ribotyping and multiplex pcr analysis of yersinia enterocolitica strains isolated from meat food in san luis argentina.the characterization of phenotypic and genotypic virulence markers of yersinia enterocolitica strains belonging to biotypes (b) 1a, 2 and 3, mostly isolated from food in san luis, argentina, and the assessment of their genotypic diversity using pfge and pcr ribotyping, were performed in our laboratory for the first time. thirty five y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied. the presence of virf, ail, ysta, and myfa genes was investig ...201021056771
[yersinia enterocolitica strains of bovine origin isolated in san luis, argentina: virulence, enterotoxigenicity, and antibiotic sensitivity].eleven yersinia enterocolitica strains isolated from bovine tongues and classified as b1 0:5 lis xz (8), b2 0:9 lis x3 (2), nontypable (1), were examined for the following virulence-associated characteristics: autoagglutination, calcium dependency, sereny test, congo red binding and presence of 40-48 mda plasmids. heat-stable enterotoxin production was also determined. antibiotic susceptibility was assayed according to the modified bauer-kirby's method. two y. enterocolitica b1 0:5 lis xz strain ...19911670473
detection of yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested pcr.the prevalence of yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (a), enrichment in modified rappaport broth at 25 °c (b), concentration by immunomagnetic separation (c) and yada nested pcr (d). furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (pfge). a total of 238 samples were collected at r ...201122265296
yersinia ssp. in surface water in san luis, argentina.yersinia spp. was examined in three rivers and two lakes located in the province of san luis, argentina, over a 1-year period. water samples were concentrated either by moore's gauze technique or by filtering through diatomaceous earth. five enrichment media: yeast extract--bengal rose broth (yer) with bile-oxalate-sorbose broth (bos); 67 mmol/l phosphate-buffered saline (ph 7.6; pbs); pbs enriched with a 1% mannitol and 1% peptone (pbsmp); pbs with lyzed 0.5% sheep blood (pbsb); wauters broth ( ...19948549992
presence of enterotoxigenic staphylococcus aureus in artisan fruit salads in the city of san luis, argentina.an increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. in this work, the microbiological quality of artisan fruit salads was analysed. faecal coliforms, salmonella spp, shigella spp, yersinia enterocolitica and escherichia coli o157:h7 were not detected; nevertheless, eleven strains of staphylococcus aureus were isolated. by multiplex pcr, all isolates showed positive results for s. aureus 16s rrna gene and 63.6% ...201424688505
genotypic and phenotypic characteristics of yersinia enterocolitica isolated from the surface of chicken eggshells obtained in argentina.the prevalence of yersinia enterocolitica on chicken eggshell surfaces in san luis, argentina, was investigated. the pathogenic potential of recovered isolates was assessed by means of phenotypic virulence tests and the presence of the 72-kb pyv plasmid. antimicrobial susceptibility was determined by the agar diffusion method. dna digested with xbai was analyzed by pulsed-field gel electrophoresis (pfge), and relationships between genomic dna profiles were established. eight y. enterocolitica b2 ...200516161678
evaluation of the pathogenic potential, antimicrobial susceptibility, and genomic relations of yersinia enterocolitica strains from food and human origin.yersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. this study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of y. enterocolitica strains of different bioserotypes (b/o) isolated from foods and human samples in san luis, argentina. strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, an ...201526370735
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