| the effect of prolonged storage on the virulence of isolates of bacillus anthracis obtained from environmental and animal sources in the kars region of turkey. | the stability of the plasmid-mediated virulence factors of bacillus anthracis, a tripartite toxin located on pxo1 and an antiphagocytic capsule encoded by genes located on pxo2, following long-term storage was investigated. a collection of 159 isolates of b. anthracis were collected from the kars region of turkey between 2000 and 2013 and stored at -20°c in brucella broth supplemented with 20% glycerine. a total of 142 isolates were recovered of which one failed to express a capsule upon primary ... | 2015 | 26109359 |